Related Experiment Video
Updated: Oct 9, 2025
A Syngeneic Orthotopic Osteosarcoma Sprague Dawley Rat Model with Amputation to Control Metastasis Rate
Published on: May 3, 2021
Ginsenoside Rg3 inhibits osteosarcoma progression by reducing circ_0003074 expression in a
Tehasi Wang1, Chengguang Zhang1, Shuren Wang2
1Graduate School, Heilongjiang University of Traditional Chinese Medicine, Harbin, Heilongjiang, China.
Background:
Previous data have suggested that ginsenoside Rg3 (Rg3), isolated from the roots of Panax ginseng, plays a repressing role in multiple cancers, including osteosarcoma (OS). However, there is no any literature available about the role of circular RNA (circRNA) in Rg3-mediated OS development. The study aimed to explore the function of circ_0003074 in the anti-cancer effects of Rg3 on OS.
Methods:
RNA expression of circ_0003074, miR-516b-5p and karyopherin subunit alpha 4 (KPNA4) was detected by quantitative real-time polymerase chain reaction (qRT-PCR). Protein expression was evaluated by Western blotting or immunohistochemistry assay. Cell viability, proliferation, apoptosis, migration and invasion were investigated by cell counting kit-8, 5-ethynyl-29-deoxyuridine (EdU), flow cytometry analysis, wound-healing and transwell invasion assays, respectively. Dual-luciferase reporter and/or RNA immunoprecipitation assay was performed to confirm the interplay between miR-516b-5p and circ_0003074 or KPNA4. Xenograft mouse model assay was conducted to reveal the effect of Rg3 treatment on tumor formation.
Results:
Circ_0003074 and KPNA4 expression was significantly upregulated, while miR-516b-5p was downregulated in OS tissues and cells compared with controls. Rg3 treatment dramatically decreased circ_0003074 expression in OS cells. Rg3 treatment led to decreased cell proliferation, migration and invasion but increased cell apoptosis, which was attenuated after circ_0003074 overexpression. Besides, miR-516b-5p was a target miRNA of circ_0003074 and partially restored circ_0003074-mediated action under Rg3 treatment. Decreasing miR-516b-5p expression also promoted Rg3-treated OS cell malignancy through KPNA4, which was identified as a target mRNA of miR-516b-5p. Besides, circ_0003074 induced KPNA4 production owing to the decrease of miR-516b-5p expression. Furthermore, Rg3 treatment inhibited tumor formation by regulating circ_0003074 in vivo.
Conclusion:
Rg3 inhibited OS progression through circ_0003074/miR-516b-5p/KPNA4 axis, showing the potential of Rg3 as a therapeutic agent for OS.
Insights
Ginsenoside Rg3 inhibits osteosarcoma (OS) progression by targeting circ_0003074, miR-516b-5p, and KPNA4. This study reveals Rg3
Area of Science:
- Oncology
- Molecular Biology
- Biochemistry
Background:
- Ginsenoside Rg3 (Rg3) shows anti-cancer properties in osteosarcoma (OS).
- The role of circular RNA (circRNA) in Rg3's effects on OS remains unexplored.
- This study investigates circ_0003074's function in Rg3-treated OS.
Purpose of the Study:
- To elucidate the mechanism of Rg3's anti-cancer activity in OS.
- To determine the role of circ_0003074 in Rg3-mediated OS progression.
- To investigate the circ_0003074/miR-516b-5p/KPNA4 axis in OS.
Main Methods:
- Quantitative real-time PCR and Western blotting for gene and protein expression analysis.
- Cellular assays (viability, proliferation, apoptosis, migration, invasion) to assess Rg3 and circ_0003074 effects.
- Dual-luciferase reporter and RNA immunoprecipitation assays to confirm molecular interactions.
- Xenograft mouse models to evaluate Rg3's in vivo anti-tumor efficacy.
Main Results:
- Circ_0003074 and KPNA4 were upregulated, while miR-516b-5p was downregulated in OS tissues.
- Rg3 treatment decreased circ_0003074 expression, suppressed proliferation, migration, and invasion, and induced apoptosis in OS cells.
- Circ_0003074 mediated these effects, with miR-516b-5p acting as a target of circ_0003074 and KPNA4 as a target of miR-516b-5p.
- Rg3 inhibited tumor formation in vivo by regulating circ_0003074.
Conclusions:
- Rg3 exerts anti-cancer effects in OS by modulating the circ_0003074/miR-516b-5p/KPNA4 pathway.
- Circ_0003074 acts as an oncogene in OS, promoting proliferation, migration, and invasion.
- Rg3 demonstrates potential as a therapeutic agent for osteosarcoma.
More Related Videos
09:03Establishment of Hepatocarcinoma in BALB/c-nu Mice and Investigation of the Therapeutic Effect of the Sanleng Jiashen Formula
Published on: January 26, 2024
04:25Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models
Published on: October 28, 2021
Related Concept Videos
01:20The Retinoblastoma Gene
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
03:03mTOR Signaling and Cancer Progression
The mTOR pathway or the...