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[A study on a functional difference in the mouse, rat and human I subregion products involved in the T cell
A functional difference in the I subregion products involved in the T cell proliferative responses to PPD was studied using monoclonal antibodies, including HOK7, ISCR3 and 1B7, which detected class II antigens across the species barrier. HOK7 detected a polymorphic determinant on I-Ab molecules, D-2.7 (rat I-E equivalent) molecules and a monomorphic determinant on DR (human I-E equivalent) molecules. ISCR3 detected a monomorphic determinant on I-E molecules, D (rat I-E equivalent) molecules and DR (human I-E equivalent) molecules. 1B7 detected a polymorphic determinant on I-Ak molecules and a monomorphic determinant on B (rat I-A equivalent) molecules. It was demonstrated that the PPD specific proliferative responses of murine T cells were reduced by HOK7 or 1B7, while ISCR3 had no significant effects on these responses. In a rat system, however, the T cell proliferative responses to PPD were inhibited by HOK7 and ISCR3, but not by 1B7. Similarly, in a human system, the T cell proliferative responses to PPD were inhibited by HOK7 and ISCR3. These results indicate the interspecies difference in function of the I subregion products involved in the T cell proliferative responses. That is the restriction molecules in these responses of mice were mainly I-A molecules, whereas those of rats and humans were mainly I-E equivalent (D and DR) molecules.
A functional difference in the I subregion products involved in the T cell proliferative responses to PPD was studied using monoclonal antibodies, including HOK7, ISCR3 and 1B7, which detected class II antigens across the species barrier. HOK7 detected a polymorphic determinant on I-Ab molecules, D-2.7 (rat I-E equivalent) molecules and a monomorphic determinant on DR (human I-E equivalent) molecules. ISCR3 detected a monomorphic determinant on I-E molecules, D (rat I-E equivalent) molecules and DR (human I-E equivalent) molecules. 1B7 detected a polymorphic determinant on I-Ak molecules and a monomorphic determinant on B (rat I-A equivalent) molecules. It was demonstrated that the PPD specific proliferative responses of murine T cells were reduced by HOK7 or 1B7, while ISCR3 had no significant effects on these responses. In a rat system, however, the T cell proliferative responses to PPD were inhibited by HOK7 and ISCR3, but not by 1B7. Similarly, in a human system, the T cell proliferative responses to PPD were inhibited by HOK7 and ISCR3. These results indicate the interspecies difference in function of the I subregion products involved in the T cell proliferative responses. That is the restriction molecules in these responses of mice were mainly I-A molecules, whereas those of rats and humans were mainly I-E equivalent (D and DR) molecules.