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Updated: Oct 9, 2025

A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses
Published on: January 25, 2018
AP2/ERF transcription factor GmDREB1 confers drought tolerance in transgenic soybean by interacting with GmERFs
Kai Chen1, Wensi Tang1, Yongbin Zhou1
1Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS)/National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Beijing, 100081, China.
Abstract:
Soybean is the main economic crop, and also the main source of oil and protein for human consumption. Drought stress has a great influence on the growth and yield of soybean crops. Therefore, improving the drought resistance of soybean, especially drought resistance in the field, is important to increase soybean yield. AP2/ERF (APETALA2/ethylene responsive factor) transcription factors are one of the largest families of transcription factors in plants. However, there has been little research on the value of applying DREB (dehydration-responsive element-binding)-like genes in improving the drought resistance of soybean. Here, we further study the value of the application of GmDREB1 in soybean. The results of drought resistance identification in the field and greenhouse showed that the overexpression of GmDREB1 could significantly enhance the drought resistance of transgenic soybean, and the yield was clearly higher than that of the wild type. GmDREB1 has transcriptional activity and is located in the nucleus. For mechanism analysis of GmDREB1 in soybean, two ERF-like transcription factors, GmERF008 and GmERF106, were shown to interact with GmDREB1 using yeast two-hybrid (Y2H) and bimolecular fluorescence complementary (BiFC) experiments. qRT-PCR (quantitative real-time PCR) results showed that the expression of many stress-related genes in GmDREB1 transgenic soybean were significantly up-regulated compared with the WT under a drought environment. In conclusion, GmDREB1 can regulate the expression of downstream stress-related genes by forming a heterodimer with ERF-like transcription factors, which can improve the drought resistance of transgenic soybean.
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Transcription
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
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