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Related Concept Videos

Mesenchymal Stem Cells01:19

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Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...
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The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
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Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
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Human mesenchymal stem cells.

Xiaoyong Chen1, Jing Huang1, Jun Wu2,3,4,5

  • 1Center for Stem Cell Biology and Tissue Engineering, Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education, Sun Yat-Sen University, Guangzhou, China.

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|December 22, 2021
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Summary
This summary is machine-generated.

New guidelines address mesenchymal stem cell (MSC) heterogeneity for cell therapy. These standards aim to improve quality control and promote international standardization for human MSCs in clinical applications.

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Area of Science:

  • Stem Cell Biology
  • Regenerative Medicine
  • Biotechnology

Background:

  • Mesenchymal stem cells (MSCs) show promise for cell therapy and tissue regeneration due to self-renewal, multipotency, and immunomodulatory functions.
  • Significant heterogeneity among MSCs from different sources or individuals hinders their clinical translation, impacting transcriptomic profiles, differentiation potential, and biological functions.
  • A critical need exists for standardized quality control and technical specifications for MSCs to ensure consistent therapeutic outcomes.

Purpose of the Study:

  • To introduce the "Human Mesenchymal Stem Cells" guidelines, a new set of standards developed in China.
  • To establish consensus on technical requirements, testing methods, and handling protocols for human MSCs (hMSCs).
  • To facilitate the adoption of uniform protocols for hMSC quality control, accelerating clinical development and therapeutic applications.

Main Methods:

  • Development of comprehensive guidelines by experts from the Chinese Society for Stem Cell Research.
  • Specification of technical requirements, test methods, regulations, usage instructions, labeling, packaging, storage, transportation, and waste disposal for hMSCs.
  • Release of the standard by the China Society for Cell Biology on January 9, 2021.

Main Results:

  • Publication of the "Human Mesenchymal Stem Cells" guidelines, providing a framework for hMSC quality control.
  • Detailed specifications covering the entire lifecycle of hMSCs, from handling to disposal.
  • Establishment of a national standard to address MSC heterogeneity and improve therapeutic consistency.

Conclusions:

  • The "Human Mesenchymal Stem Cells" guidelines provide essential technical specifications for hMSC quality control.
  • These guidelines are expected to promote the institutional adoption of standardized protocols for hMSC research and clinical use.
  • The initiative aims to accelerate the international standardization of hMSCs, fostering their successful translation into clinical therapies.