Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Tagging and Fusion Proteins

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Self-Assembling Cationic Lipopeptides for the Construction of Functional Vesicular Gene-Delivery Systems.

Small (Weinheim an der Bergstrasse, Germany)·2026

Same author

Achieving cell-type-specific bioorthogonal chemistry using enzyme-activated caged tetrazines.

Nature chemical biology·2026

Same author

Breaking the membrane heredity paradox through de novo protocell formation.

Nature communications·2026

Same author

<i>β</i>-Sheet Structures Enhance Aggregation-Induced Emission Signals in Peptide-Based Coacervates.

Chemistry of materials : a publication of the American Chemical Society·2026

Same author

Enzyme-Mediated Covalent Labeling Enables In Situ Imaging of RNA Modification States.

Journal of the American Chemical Society·2026

Same author

Harnessing S. aureus tRNA Guanine Transglycosylase for Efficient Site-Specific Covalent Labeling of DNA.

Chembiochem : a European journal of chemical biology·2026

Same journal

Reaction Optimization for Enzymatic Deconstruction of Industrially Relevant Nylon Composites.

Chembiochem : a European journal of chemical biology·2026

Same journal

Deploying Artificial Metalloenzymes in Complex Environments: Strategies and Applications.

Chembiochem : a European journal of chemical biology·2026

Same journal

Synthetic Ligands of Myeloid C-Type Lectin Receptors.

Chembiochem : a European journal of chemical biology·2026

Same journal

Vancomycin-Mediated Binding of DNA Origami Nanostructures to Gram-Positive and Gram-Negative Bacteria.

Chembiochem : a European journal of chemical biology·2026

Same journal

Mutasynthesis and Antibiotic Activity of Mupirocin Analogues.

Chembiochem : a European journal of chemical biology·2026

Same journal

Pressure-Dependent Aromatic Ring Flips Reveal Variable Transition-State Volume and Compressibility Among Structural Regions of BPTI.

Chembiochem : a European journal of chemical biology·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Oct 9, 2025

SNARE-mediated Fusion of Single Proteoliposomes with Tethered Supported Bilayers in a Microfluidic Flow Cell Monitored by Polarized TIRF Microscopy

SNARE-mediated Fusion of Single Proteoliposomes with Tethered Supported Bilayers in a Microfluidic Flow Cell Monitored by Polarized TIRF Microscopy

Published on: August 24, 2016

Controlling Protein Enrichment in Lipid Sponge Phase Droplets using SNAP-Tag Bioconjugation.

Mahta Moinpour¹, Alessandro Fracassi¹, Roberto J Brea²

¹Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, Natural Sciences Building 3328, La Jolla, CA 92093, USA.

Chembiochem : a European Journal of Chemical Biology

|December 22, 2021

Summary

This summary is machine-generated.

Scientists developed a new method to create artificial cell compartments using lipid sponge droplets. This system allows precise protein capture for advanced artificial organelles.

Keywords:

SNAP-tag artificial organelles lipids sponge phase synthetic biology

More Related Videos

Inducible LAP-tagged Stable Cell Lines for Investigating Protein Function, Spatiotemporal Localization and Protein Interaction Networks

Inducible LAP-tagged Stable Cell Lines for Investigating Protein Function, Spatiotemporal Localization and Protein Interaction Networks

Published on: December 24, 2016

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis

Published on: April 17, 2017

Related Experiment Videos

Last Updated: Oct 9, 2025

SNARE-mediated Fusion of Single Proteoliposomes with Tethered Supported Bilayers in a Microfluidic Flow Cell Monitored by Polarized TIRF Microscopy

SNARE-mediated Fusion of Single Proteoliposomes with Tethered Supported Bilayers in a Microfluidic Flow Cell Monitored by Polarized TIRF Microscopy

Published on: August 24, 2016

Inducible LAP-tagged Stable Cell Lines for Investigating Protein Function, Spatiotemporal Localization and Protein Interaction Networks

Inducible LAP-tagged Stable Cell Lines for Investigating Protein Function, Spatiotemporal Localization and Protein Interaction Networks

Published on: December 24, 2016

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis

Published on: April 17, 2017

Area of Science:

Biotechnology and Synthetic Biology
Cellular and Molecular Biology
Materials Science

Background:

Cells utilize membrane-bound organelles for specialized functions, creating unique chemical environments.
Developing functional artificial lipid-based compartments remains a significant challenge in synthetic biology.

Purpose of the Study:

To establish a robust bioconjugation system for sequestering proteins into artificial lipid compartments.
To enable programmable control over protein incorporation within these compartments for advanced applications.

Main Methods:

Utilized zwitterionic lipid sponge phase droplets as the basis for artificial compartments.
Incorporated benzylguanine (BG)-modified phospholipids for covalent linkage with SNAP-tag fusion proteins.
Employed O6-methylguanine DNA methyltransferase (SNAP-tag) technology for specific protein capture.

Main Results:

Successfully demonstrated a bioconjugation system for sequestering proteins into lipid sponge droplets.
Achieved programmable control of protein capture via the SNAP-tag and BG-modified phospholipid interaction.
Anchored hydrophilic proteins at the lipid-aqueous interface, creating a protected and accessible chemical environment.

Conclusions:

The developed methodology provides a novel approach for creating functional artificial lipid-based compartments.
SNAP-tag technology facilitates the integration of functional proteins, paving the way for advanced artificial organelles.
This system offers a promising platform for future research in synthetic cell biology and biomimetic systems.