A mitochondrial and lysosomal targeted ratiometric probe for detecting intracellular H₂S

Based on coumarin and benzopyran derivatives, a dual-wavelength excitation ratiometric fluorescent probe, HABA, was prepared to detect H₂S. The HABA probe showed good selectivity and anti-interference abilities during H₂S detection. Fluorescence co-localization experiments showed that HABA had excellent localization abilities toward mitochondria and lysosomes. More importantly, HABA can not only detect exogenous H₂S, but it can also detect endogenous H₂S, indicating that HABA has high application potential and value in the biological field.