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iChip

iChip

The cultivation of environmental microorganisms has long been hindered by the inability to replicate complex native conditions in vitro. The isolation chip (iChip) addresses this limitation by facilitating the growth of previously uncultivable microorganisms through in situ incubation. Designed for high-throughput microbial cultivation, the iChip comprises hundreds of microchambers, each capable of housing a single microbial cell. These microchambers are loaded with a mixture of molten agar and...

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Related Experiment Video

Updated: Jun 10, 2026

Co-culture of Living Microbiome with Microengineered Human Intestinal Villi in a Gut-on-a-Chip Microfluidic Device

Co-culture of Living Microbiome with Microengineered Human Intestinal Villi in a Gut-on-a-Chip Microfluidic Device

Published on: August 30, 2016

Intestinal Stem Cell-on-Chip to Study Human Host-Microbiota Interaction.

Fatina Siwczak¹, Elise Loffet², Mathilda Kaminska¹

¹Center for Sepsis Control and Care & Institute of Biochemistry II, University Hospital Jena, Jena, Germany.

Frontiers in Immunology

|December 23, 2021

Summary

This summary is machine-generated.

Organ-on-chip technology enables precise control over intestinal epithelial cell differentiation in vitro. This approach aids in studying gut development, tissue formation, and microbiota interactions for gastrointestinal disease research.

Keywords:

gut-on-chip host-microbiota interaction in vitro model intestine microbiota stem cell

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Last Updated: Jun 10, 2026

Co-culture of Living Microbiome with Microengineered Human Intestinal Villi in a Gut-on-a-Chip Microfluidic Device

Co-culture of Living Microbiome with Microengineered Human Intestinal Villi in a Gut-on-a-Chip Microfluidic Device

Published on: August 30, 2016

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Published on: July 28, 2023

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Published on: May 24, 2024

Area of Science:

Gastroenterology
Developmental Biology
Biotechnology

Background:

The gut epithelium, crucial for nutrient absorption and pathogen defense, comprises specialized cells organized in crypts and villi.
Intestinal stem cell differentiation and self-renewal are regulated by niche signals along the crypt-villus axis.
Microphysiological systems, or organ-on-chips, offer dynamic, controlled environments for studying intestinal epithelium.

Purpose of the Study:

To review the application of organ-on-chip technology for controlling intestinal epithelial differentiation in vitro.
To discuss current and future strategies for investigating stem cell differentiation, tissue formation, and host-microbiota interactions using these systems.
To explore the role of mechanical processes in intestinal development and disease.

Main Methods:

Utilizing microphysiological systems (organ-on-chips) to create controlled in vitro models of the intestinal epithelium.
Manipulating niche signals within the organ-on-chip environment to guide cell differentiation.
Analyzing tissue formation, cell behavior, and host-microbiota interactions within the engineered gut model.

Main Results:

Organ-on-chip technology allows for precise control over the differentiation of intestinal epithelial cells.
These systems facilitate the study of mechanical forces influencing stem cell behavior and tissue development.
The technology enables investigation of the complex interplay between the intestinal epithelium and the microbiome.

Conclusions:

Organ-on-chip technology is a powerful tool for dissecting the mechanisms of intestinal development and disease.
This approach provides novel strategies for understanding stem cell differentiation and tissue regeneration in the gut.
Future applications include modeling gastrointestinal diseases and evaluating therapeutic interventions by studying gut-microbiota interactions.