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Related Experiment Video

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Quantifying Cyanothece growth under DIC limitation.

Keisuke Inomura1, Takako Masuda2, Meri Eichner2

  • 1Graduate School of Oceanography, University of Rhode Island, Narragansett, Rhode Island, USA.

Computational and Structural Biotechnology Journal
|December 23, 2021
PubMed
Summary
This summary is machine-generated.

Dissolved inorganic carbon (DIC) limits growth in the model organism Cyanothece. High carbon storage for nitrogen fixation depletes DIC, impacting photosynthesis and cellular growth rates.

Keywords:
BiomassCO2CarbonCarbon allocationCarbon storageCellular growthComputer simulationCultureCyanotheceDICDiurnal cycleGrowth limitationMathematical modelNitrateNitrogen fixationPhotosynthesisQuantitative modelTurbidostat

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Area of Science:

  • Microbial Ecology
  • Photosynthesis Research
  • Biogeochemical Cycles

Background:

  • Cyanothece is a model organism for studying photosynthesis and nitrogen fixation.
  • Temporal segregation of carbon and nitrogen fixation occurs in its diel cycle.
  • Understanding growth limitations is crucial for aquatic ecosystem modeling.

Purpose of the Study:

  • To investigate dissolved inorganic carbon (DIC) as a limiting factor for Cyanothece growth.
  • To develop a quantitative model linking DIC concentration to Cyanothece growth.
  • To explore the role of intracellular carbon allocation in growth rate.

Main Methods:

  • A simple quantitative model was developed.
  • Experimental data from laboratory studies were utilized.
  • Measurements of DIC consumption, photosynthesis rates, and carbon accumulation were performed.

Main Results:

  • External DIC concentration was identified as a major growth-limiting factor for Cyanothece.
  • Rapid DIC consumption by photosynthesis limited carbon accumulation and growth.
  • Nitrogen fixation requires significant carbon storage, reducing carbon available for growth.

Conclusions:

  • Cyanothece growth is significantly limited by DIC availability due to high carbon storage demands.
  • Intracellular carbon allocation strategies critically influence growth rates.
  • The model provides a framework for incorporating DIC limitation in aquatic ecosystem simulations.