Field sampling of Anopheles mosquitoes for correlated cytogenetic, electrophoretic and morphological studies

Anopheline mosquitos were preserved in liquid nitrogen for up to 8 months prior to dissection and preparation of ovarian polytene chromosomes. The 23 preparations examined all had excellent chromosomes. A method is described for the preservation of the wings, legs and palps of these mosquitos as museum specimens for taxonomic studies; the remaining tissues from the frozen specimens can then be used for electrophoresis. Blood meal identification can be carried out on the undigested stomach contents.