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Updated: Oct 9, 2025

Digital Analysis of Immunostaining of ZW10 Interacting Protein in Human Lung Tissues
Published on: May 1, 2019
ZO-1 Intracellular Localization Organizes Immune Response in Non-Small Cell Lung Cancer
Déborah Neyrinck-Leglantier1, Julien Lesage1,2, Silvia Blacher3
1University of Reims Champagne-Ardenne, Inserm UMR-S 1250, SFR CAP-Santé, Reims, France.
Abstract:
Delocalization of zonula occludens-1 (ZO-1) from tight junctions plays a substantial role in epithelial cell plasticity observed during tumor progression. In vitro, we reported an impact of ZO-1 cyto-nuclear content in modulating the secretion of several pro-inflammatory chemokines. In vivo, we demonstrated that it promotes the recruitment of immune cells in mouse ear sponge assays. Examining lung cancers, we showed that a high density of CD8 cytotoxic T cells and Foxp3 immunosuppressive regulatory T cells in the tumor microenvironment correlated with a cyto-nuclear expression of ZO-1. Taken together, our results support that, by affecting tumor cell secretome, the cyto-nuclear ZO-1 pool may recruit immune cells, which could be permissive for tumor progression.
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