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Grape Lipidomics: An Extensive Profiling thorough UHPLC-MS/MS Method.

Domenico Masuero1, Domen Škrab1,2, Giulia Chitarrini1

  • 1Research and Innovation Centre, Department of Food Quality and Nutrition, Fondazione Edmund Mach, 38098 San Michele all'Adige, Italy.

Metabolites
|December 23, 2021
PubMed
Summary
This summary is machine-generated.

This study developed an ultrahigh performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) method for analyzing grape lipids. The method successfully profiled 412 lipid compounds and revealed associations between specific lipids and grape maturation indicators like °Brix.

Keywords:
grapelipidomelipidomicsliquid chromatographymass spectrometry

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Area of Science:

  • * Plant lipidomics
  • * Analytical chemistry
  • * Biochemistry

Background:

  • * Lipids are vital amphiphilic molecules with diverse roles and compositions influenced by various factors.
  • * Liquid chromatography-mass spectrometry (LC-MS) is increasingly used for plant lipid analysis due to advances in mass spectrometry.
  • * Comprehensive studies on grape lipid composition remain limited.

Purpose of the Study:

  • * To develop and validate an ultrahigh performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) method for grape lipid profiling.
  • * To analyze the lipid profile variations during the maturation of Ribolla Gialla cv. grapes.
  • * To identify associations between specific lipid classes and grape maturation indicators.

Main Methods:

  • * Development of a UHPLC-MS/MS method employing 8098 Multiple Reaction Monitoring (MRM) transitions.
  • * Validation of the method using a reference grape sample at maturity, enabling semi-quantification of 412 compounds.
  • * Application of the validated method to study lipid profile changes during grape ripening and analysis using partial least squares (PLS) regression.

Main Results:

  • * Successful semi-quantification of 412 distinct lipid compounds in grapes.
  • * Identification of positive associations between increasing °Brix and higher proportions of glycerophospholipids (GPE, PG) and their hydrolysates (LPC, LPE).
  • * Negative associations were observed between increasing °Brix and ceramides (CER) and digalactosyldiacylglycerols (DGDG).

Conclusions:

  • * The developed UHPLC-MS/MS method is robust and effective for comprehensive grape lipid profiling.
  • * The study provides insights into lipid dynamics during grape maturation, linking specific lipids to °Brix levels.
  • * The method holds potential for broader applications in lipidomics research across different matrices.