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Phase Contrast and Differential Interference Contrast Microscopy

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

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Related Experiment Video

Updated: Oct 9, 2025

Multimodal Quantitative Phase Imaging with Digital Holographic Microscopy Accurately Assesses Intestinal Inflammation and Epithelial Wound Healing

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Roadmap on Digital Holography-Based Quantitative Phase Imaging.

Vinoth Balasubramani^1,2, Małgorzata Kujawińska³, Cédric Allier⁴

¹Division of Biological and Environmental Sciences and Engineering, King Adullah University of Science and Technology, Thuwal 23955-6900, Saudi Arabia.

Journal of Imaging

|December 23, 2021

Summary

This summary is machine-generated.

Quantitative Phase Imaging (QPI) offers advanced microscopy for microstructures. This review covers digital holography QPI methods and future applications in 2D and 3D imaging.

Keywords:

3D distribution of refractive index biomedical analysis at cellular level digital holographic microscopy holographic tomography quantitative phase imaging

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Area of Science:

Optics and Photonics
Biomedical Imaging
Microscopy

Background:

Quantitative Phase Imaging (QPI) integrates microscopy, interferometry, holography, and computation.
It enables advanced imaging of biological and technical microstructures.

Purpose of the Study:

To review digital holography-based QPI approaches.
To discuss current and future perspectives of 2D and 3D QPI.

Main Methods:

Review of prominent research on digital holographic microscopy.
Exploration of holographic tomography techniques.

Main Results:

Compilation of various digital holography-based QPI methods.
Overview of established and emerging QPI applications.

Conclusions:

Digital holography-based QPI is a powerful technique for microstructure analysis.
Future research will expand 2D and 3D QPI capabilities and applications.