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Updated: Oct 9, 2025
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Real-time Live Imaging of T-cell Signaling Complex Formation
Published on: June 23, 2013
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Membrane prohibitin forms a dynamic complex with p56lck to regulate T cell receptor signaling
Debjani Dutta1, Srikanth K Santhanam1, Farhat Parween1
1Hybridoma Laboratory, National Institute of Immunology, Aruna Asaf ali marg, New Delhi, 110067 India.
Immunology Letters
|December 23, 2021
Summary
Salmonella Typhi
Area of Science:
- Immunology
- Cellular Biology
- Molecular Biology
Background:
- Prohibitin is a conserved protein vital for cellular functions.
- The Vi polysaccharide from Salmonella Typhi suppresses T cell IL-2 secretion.
- The mechanism behind this T cell suppression by Vi remains unclear.
Purpose of the Study:
- To elucidate the mechanism by which Vi polysaccharide suppresses T cell receptor (TCR) signaling.
- To investigate the role of membrane prohibitin in T cell activation.
Main Methods:
- Utilized Jurkat cells, a human T cell line, as a model system.
- Employed Vi polysaccharide as a molecular probe.
- Investigated protein-protein interactions using co-immunoprecipitation and analyzed signaling pathways (Lck, ERK) via Western blotting.
Main Results:
- Demonstrated that membrane prohibitin associates with p56lck (Lck) and actin in Jurkat cells.
- Showed that anti-CD3 antibody activation causes dissociation of the prohibitin-Lck-actin complex, coinciding with ERK activation.
- Found that Vi binding prevents Lck and ERK activation by inhibiting the dissociation of Lck from prohibitin.
Conclusions:
- Membrane prohibitin plays a regulatory role in Lck activation and proximal T cell receptor signaling.
- Vi polysaccharide from Salmonella Typhi interferes with prohibitin-mediated regulation of Lck, leading to suppressed T cell activation.
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