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Related Experiment Videos

Epidermal growth factor in blood.

A P Savage, V K Chatterjee, H Gregory

    Regulatory Peptides
    |December 30, 1986
    PubMed
    Summary

    Epidermal growth factor (EGF) circulates bound to platelets. Blood coagulation releases free EGF, indicating its role in various tissues and tumors requires further investigation of its precise concentrations.

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    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Endocrinology

    Background:

    • Epidermal Growth Factor (EGF) receptors are present in diverse human tissues and some tumors.
    • The precise role of EGF in circulation remains undefined, necessitating accurate concentration measurements in biological fluids.

    Purpose of the Study:

    • To investigate the occurrence and forms of EGF in human blood circulation.
    • To determine the influence of blood processing on EGF levels and bioavailability.

    Main Methods:

    • Quantification of EGF in platelet-rich plasma, platelet-poor plasma, and serum using immunoassays.
    • Analysis of EGF molecular weight and immunoreactivity distribution via gel chromatography.
    • Assessment of EGF levels following varying blood separation times.

    Main Results:

    • EGF levels were significantly higher in platelet-rich plasma (51 pmol/l) compared to platelet-poor plasma (2.9 pmol/l).
    • Serum EGF concentrations increased with delayed separation, from 37 pmol/l at 30 min to 117 pmol/l at 270 min.
    • EGF in platelet-poor plasma existed primarily as a high molecular weight, potentially inactive form, while serum showed increased free EGF with delayed separation.

    Conclusions:

    • Circulating EGF is predominantly associated with platelets.
    • Blood coagulation triggers the release of free, potentially biologically active EGF.
    • Understanding EGF's release mechanism is crucial for elucidating its role in human physiology and pathology.

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