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Sanguiin H-6 Fractionated from Cloudberry (Rubus chamaemorus) Seeds Can Prevent the Methicillin-Resistant
John Jairo Aguilera-Correa1,2, Sara Fernández-López3, Iskra Dennisse Cuñas-Figueroa3
1Clinical Microbiology Department, IIS-Fundación Jiménez Díaz, UAM. Avda. Reyes Católicos 2, 28040 Madrid, Spain.
Abstract:
Staphylococcus aureus is the most common cause of surgical site infections and its treatment is challenging due to the emergence of multi-drug resistant strains such as methicillin-resistant S. aureus (MRSA). Natural berry-derived compounds have shown antimicrobial potential, e.g., ellagitannins such as sanguiin H-6 and lambertianin C, the main phenolic compounds in Rubus seeds, have shown antimicrobial activity. The aim of this study was to evaluate the effect of sanguiin H-6 and lambertianin C fractionated from cloudberry seeds, on the MRSA growth, and as treatment of a MRSA biofilm development in different growth media in vitro and in vivo by using a murine wound infection model where sanguiin H-6 and lambertianin C were used to prevent the MRSA infection. Sanguiin H-6 and lambertianin C inhibited the in vitro biofilm development and growth of MRSA. Furthermore, sanguiin H-6 showed significant anti-MRSA effect in the in vivo wound model. Our study shows the possible use of sanguiin H-6 as a preventive measure in surgical sites to avoid postoperative infections, whilst lambertianin C showed no anti-MRSA activity.
Insights
Sanguiin H-6 from cloudberry seeds effectively inhibited methicillin-resistant Staphylococcus aureus (MRSA) growth and biofilm development in vitro and in vivo. This compound shows promise for preventing surgical site infections.
Area of Science:
- Natural product chemistry
- Microbiology
- Infectious diseases
Background:
- Surgical site infections (SSIs) are commonly caused by Staphylococcus aureus.
- Emergence of multi-drug resistant strains like methicillin-resistant S. aureus (MRSA) complicates treatment.
- Natural compounds, including ellagitannins from Rubus seeds, exhibit antimicrobial properties.
Purpose of the Study:
- To evaluate the anti-MRSA efficacy of sanguiin H-6 and lambertianin C from cloudberry seeds.
- To assess their impact on MRSA growth and biofilm development in vitro.
- To investigate their therapeutic potential in a murine wound infection model.
Main Methods:
- Fractionation of sanguiin H-6 and lambertianin C from cloudberry seeds.
- In vitro assessment of MRSA growth and biofilm inhibition.
- In vivo evaluation using a murine wound infection model to test preventive effects.
Main Results:
- Both sanguiin H-6 and lambertianin C inhibited in vitro MRSA growth and biofilm formation.
- Sanguiin H-6 demonstrated significant anti-MRSA activity in the in vivo wound model.
- Lambertianin C did not show significant anti-MRSA activity in this study.
Conclusions:
- Sanguiin H-6 is a potent inhibitor of MRSA growth and biofilm.
- Sanguiin H-6 holds potential as a preventive agent against SSIs.
- Further research may explore sanguiin H-6 for clinical applications in infection control.
