Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

In Vitro Fertilization

In Vitro Fertilization

In vitro fertilization (IVF) is a form of assisted reproductive technology where an egg is fertilized with sperm in a controlled laboratory environment before transferring the resulting embryo into the uterus. This process is designed to help individuals and couples experiencing difficulties conceiving.
The IVF process begins with ovarian stimulation, during which reproductive endocrinologists prescribe hormonal medications to stimulate the ovaries to produce multiple eggs instead of the single...

Meiosis II

Meiosis II

Meiosis II is the second and final stage of meiosis. It relies on the haploid cells produced during meiosis I, each of which contain only 23 chromosomes—one from each homologous initial pair. Importantly, each chromosome in these cells is composed of two joined copies, and when these cells enter meiosis II, the goal is to separate such sister chromatids using the same microtubule-based network employed in other division processes. The result of meiosis II is two haploid cells, each...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Artificial Intelligence Dystocia Algorithm (AIDA) for Risk Stratification of Occiput Posterior Fetal Head Position.

Journal of imaging·2026

Same author

Ultrasound Features of Uterine Perivascular Epithelioid Cell Tumor (PEComa): A Systematic Review.

Journal of imaging·2026

Same author

From single markers to the geometry of the cardinal movements in occiput posterior position.

European journal of obstetrics, gynecology, and reproductive biology·2026

Same author

Assessment of mycotoxin exposure in women undergoing assisted reproduction: A study of urinary biomarkers related to oocyte and embryo development.

Reproductive toxicology (Elmsford, N.Y.)·2026

Same author

Modulation of L-Type Calcium Currents by Resveratrol-Induced Myogenesis in C2C12 Cells.

Cells·2026

Same author

From Transition to Reproduction: A Narrative Review of Evolving Evidence in Transgender Obstetrics and Gynecology.

Gynecologic and obstetric investigation·2026

Search research articles

Related Experiment Video

Updated: Oct 9, 2025

U-Shaped Horizontal Swimming Technique for Preparing High-Quality Sperm with Low DNA Fragmentation Index

U-Shaped Horizontal Swimming Technique for Preparing High-Quality Sperm with Low DNA Fragmentation Index

Published on: March 28, 2025

Sperm Selection for ICSI: Do We Have a Winner?

Domenico Baldini¹, Daniele Ferri¹, Giorgio Maria Baldini¹

¹MOMO Fertilife-IVF Center, 76011 Bisceglie, Italy.

|December 24, 2021

Summary

Selecting the best sperm is crucial for assisted reproductive technology (ART) and intracytoplasmic sperm injection (ICSI). This review examines various sperm selection methods to find the most effective technique for fertilization.

Keywords:

assisted reproductive technology (ART)density gradient (DGC)horizontal sperm migration in vitro fertilization (IVF)intracytoplasmic sperm injection (ICSI)microfluidic sperm selection swim-up (SU)

More Related Videos

Mouse Round Spermatid Injection

Mouse Round Spermatid Injection

Published on: January 26, 2024

Author Spotlight: Exploring the Long-Term Health Impacts of Intracytoplasmic Sperm Injection on Offspring

Author Spotlight: Exploring the Long-Term Health Impacts of Intracytoplasmic Sperm Injection on Offspring

Published on: May 17, 2024

Related Experiment Videos

Last Updated: Oct 9, 2025

U-Shaped Horizontal Swimming Technique for Preparing High-Quality Sperm with Low DNA Fragmentation Index

U-Shaped Horizontal Swimming Technique for Preparing High-Quality Sperm with Low DNA Fragmentation Index

Published on: March 28, 2025

Mouse Round Spermatid Injection

Mouse Round Spermatid Injection

Published on: January 26, 2024

Author Spotlight: Exploring the Long-Term Health Impacts of Intracytoplasmic Sperm Injection on Offspring

Author Spotlight: Exploring the Long-Term Health Impacts of Intracytoplasmic Sperm Injection on Offspring

Published on: May 17, 2024

Area of Science:

Reproductive biology
Assisted reproductive technology (ART)

Background:

Sperm preparation is vital for successful fertilization in ART, particularly intracytoplasmic sperm injection (ICSI).
Current methods aim to mimic natural sperm selection but face ongoing debate regarding efficacy.

Purpose of the Study:

To review and compare available sperm selection techniques for ICSI.
To evaluate methods for selecting competent spermatozoa with high fertilization potential.

Main Methods:

Discussion of basic and advanced sperm selection techniques.
Examination of methods exploiting sperm membrane characteristics and microfluidics.
Analysis of a novel micro swim-up technique for direct ICSI dish application.

Main Results:

Various techniques exist, including those for sperm with reduced motility.
Microfluidics and micro swim-up represent advanced strategies.
Each method presents unique advantages and disadvantages.

Conclusions:

No definitive gold standard method for sperm selection in ICSI has been established.
Further evaluation is needed to determine the most effective technique for optimizing fertilization outcomes.