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Isolation of Human Myoblasts, Assessment of Myogenic Differentiation, and Store-operated Calcium Entry Measurement
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Modulation of L-Type Calcium Currents by Resveratrol-Induced Myogenesis in C2C12 Cells
Andrea Biagini1,2, Luana Sallicandro1,2, Jasmine Covarelli2
1Department of Chemistry, Biology and Biotechnologies, University of Perugia, Via dell'Elce di Sotto 8, 06132 Perugia, PG, Italy.
Cells
|April 13, 2026
Summary
Resveratrol (RES) promotes skeletal muscle cell maturation by altering calcium channel activity, shifting it towards an adult form. This suggests RES could be a promising treatment for neuromuscular disorders like myotonic dystrophy type 1.
Area of Science:
- Electrophysiology
- Molecular Biology
- Cellular Physiology
Background:
- Skeletal muscle differentiation relies on membrane potential and ion channel activity.
- Altered ion channel function is linked to muscle diseases like muscular dystrophy.
- C2C12 myogenesis involves complex electrophysiological changes.
Purpose of the Study:
- Investigate electrophysiological changes during C2C12 myogenesis.
- Examine resveratrol's (RES) effects on calcium channel currents (Cav1.1).
- Determine RES's potential therapeutic role in neuromuscular disorders.
Main Methods:
- Whole-cell patch-clamp recordings in C2C12 cells.
- Analysis of potassium (K+) currents and voltage-dependent calcium channels (VDCCs).
- Assessment of RES (30 µM) impact on Cav1.1 channel gating and amplitude.
Main Results:
- Identified three K+ current profiles during differentiation, correlating with membrane hyperpolarization.
- Observed nimodipine-sensitive L-type Ca2+ currents (Cav1.1) in C2C12 myocytes.
- RES exposure shifted Cav1.1 activation depolarizing and reduced current amplitude, favoring adult splice variants.
Conclusions:
- RES promotes skeletal muscle electrophysiological maturation by modulating Cav1.1 expression and gating.
- RES may restore calcium homeostasis in neuromuscular disorders by influencing CACNA1S splicing.
- Resveratrol shows promise as a therapeutic agent for conditions like myotonic dystrophy type 1 (DM1).
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