Related Experiment Video
Updated: Oct 9, 2025
09:58
DNA-barcode-based Multiplex Immunofluorescence Imaging to Analyze FFPE Specimens from Genetically Reprogrammed Murine Melanoma
Published on: June 6, 2025
553
Mapping the Melanoma Plasma Proteome (MPP) Using Single-Shot Proteomics Interfaced with the WiMT Database
Natália Almeida1,2,3, Jimmy Rodriguez4, Indira Pla Parada5
1Laboratory of Proteomics/LADETEC, Universidade Federal Do Rio de Janeiro, Rio de Janeiro 21941-598, Brazil.
Cancers
|December 24, 2021
Summary
Wise MS Transfer (WiMT) is a new proteomics method for analyzing melanoma plasma. This technique identifies over 1200 proteins, including low-abundance markers, advancing melanoma research.
Area of Science:
- Proteomics
- Mass Spectrometry
- Biomarker Discovery
Background:
- Plasma proteomics faces challenges due to a 10-order dynamic range.
- Accurate protein identification in complex samples like melanoma plasma is difficult.
Purpose of the Study:
- To develop a novel methodology for protein identification in plasma.
- To enhance the identification of low-abundance proteins in melanoma plasma samples.
Main Methods:
- Developed Wise MS Transfer (WiMT), a methodology for protein identification.
- Utilized MS1 features across multiple MS runs and custom protein databases.
- Applied single-shot proteomics with multi-level dynamic protein databases and immunodepletion strategies.
Main Results:
- Identified >1200 proteins from >10,000 protein sequences in melanoma plasma.
- Annotated over 660 proteins using WiMT, identifying 12 known candidate markers.
- Discovered low-abundant proteins involved in cell signaling, immune regulation, and protein processing.
Conclusions:
- WiMT enables simple sample preparation for large-scale screening of melanoma plasma.
- The platform facilitates the discovery of novel proteins critical to melanoma disease functions.
- WiMT significantly advances the capability for comprehensive plasma proteome analysis.
