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Related Concept Videos

Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

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The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
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lncRNA - Long Non-coding RNAs02:39

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In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
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Translational Regulation01:29

Translational Regulation

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Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
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Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

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Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
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What is Gene Expression?01:36

What is Gene Expression?

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A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then...
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Experimental RNAi02:15

Experimental RNAi

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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
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NAPRT Expression Regulation Mechanisms: Novel Functions Predicted by a Bioinformatics Approach.

Sara Duarte-Pereira1,2, Olga Fajarda2, Sérgio Matos2,3

  • 1Department of Medical Sciences, iBiMED-Institute of Biomedicine, University of Aveiro, 3810-193 Aveiro, Portugal.

Genes
|December 24, 2021
PubMed
Summary
This summary is machine-generated.

Nicotinate phosphoribosyltransferase (NAPRT) regulates NAD biosynthesis, a key target in cancer therapy. This study identifies novel regulators of NAPRT gene expression, revealing its unexpected roles in development and neuronal biology.

Keywords:
NAPRT (nicotinate phosphoribosyltransferase)bioinformaticscell differentiationexpression regulationneurodevelopment

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Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Nicotinate phosphoribosyltransferase (NAPRT) is crucial for NAD biosynthesis, making it a relevant target for cancer therapeutics.
  • NAD metabolism is vital for proliferating cancer cells, and NAPRT expression varies across cancer types, necessitating pre-therapeutic assessment.
  • The extracellular form of NAPRT (eNAPRT) suggests roles beyond NAD synthesis, including inflammation and signaling.

Purpose of the Study:

  • To investigate the regulatory mechanisms governing NAPRT gene expression.
  • To identify transcription factors (TFs), RNA binding proteins (RBPs), and microRNAs (miRNAs) that control NAPRT transcription, alternative splicing, and expression levels.

Main Methods:

  • Bioinformatic analysis of TF, RBP, and miRNA databases.
  • Gene Ontology (GO) enrichment analysis.
  • Expression profiling analysis.

Main Results:

  • Several potential regulators of NAPRT transcription activation, downregulation, and alternative splicing were identified.
  • Functional analysis revealed novel associations between NAPRT and cell differentiation, development, and neuronal biology.
  • The study provides a comprehensive overview of NAPRT gene expression regulation.

Conclusions:

  • The identified regulatory mechanisms offer new insights into NAPRT's role in cellular processes.
  • These findings suggest potential new therapeutic strategies targeting NAPRT beyond its role in NAD metabolism.
  • NAPRT's involvement in cell differentiation, development, and neuronal biology opens new avenues for research.