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Related Experiment Video

Updated: Oct 9, 2025

Measuring Cell Cycle Progression Kinetics with Metabolic Labeling and Flow Cytometry

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Published on: May 22, 2012

The CWI Pathway: A Versatile Toolbox to Arrest Cell-Cycle Progression.

Inma Quilis¹, Mercè Gomar-Alba¹, Juan Carlos Igual¹

¹Departament de Bioquímica i Biologia Molecular, Institut Universitari de Biotecnologia i Biomedicina (BIOTECMED), Universitat de València, 46100 València, Spain.

Journal of Fungi (Basel, Switzerland)

|December 24, 2021

Summary

The cell-wall integrity (CWI) pathway in yeast acts as a crucial brake, halting cell-cycle progression during environmental stress. This pathway ensures cell survival by coordinating responses to damage and maintaining genetic integrity.

Keywords:

DNA damage Pkc1 Slt2 cell cycle cell wall integrity checkpoint

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Area of Science:

Cell Biology
Molecular Biology
Genetics

Background:

Cell-signaling pathways enable cells to adapt to environmental changes.
The cell-wall integrity (CWI) pathway in *Saccharomyces cerevisiae* responds to cell wall damage and stress.
CWI pathway activation is linked to cell-cycle progression and polarized growth.

Purpose of the Study:

To elucidate the role of the CWI pathway in regulating cell-cycle transitions.
To investigate how CWI pathway signaling impacts cell-cycle progression in response to cell surface perturbation.
To explore the connection between the CWI pathway, genotoxic stress, and DNA integrity checkpoints.

Main Methods:

Analysis of cell-cycle progression under various stress conditions.
Investigating the regulatory mechanisms of the CWI pathway on cell-cycle transitions.
Examining the interplay between CWI signaling and DNA integrity checkpoints.

Main Results:

The CWI pathway delays cell-cycle progression at critical transitions like G1/S and mitosis.
It regulates the Start transcriptional program and DNA replication initiation.
The pathway connects to the DNA integrity checkpoint, crucial for cell survival under genotoxic stress.

Conclusions:

The CWI pathway acts as a master regulator, halting cell-cycle progression during unfavorable conditions.
It plays a vital role in maintaining cellular homeostasis and survival by integrating stress responses with cell-cycle control.
This pathway is essential for preventing the propagation of damaged cells.