Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

238
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
238
Flow Cytometry01:23

Flow Cytometry

14.2K
The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...
14.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Single-cell detection and quantification of the microbiota by MicFLY.

Cell host & microbe·2026
Same author

Microbial metabolites at the front line: Orchestrating gastrointestinal and systemic barrier immunity across the lifespan.

Cell reports·2026
Same author

Stable charged nanobubbles with distinct polarities in culture media differentially affect the viability of human iPSC-derived neurons.

Scientific reports·2026
Same author

Microbial succession at weaning is guided by microbial metabolism of host glycans.

Microbiome·2025
Same author

Single-cell quantification of the microbiota by flow cytometry: MicFLY.

bioRxiv : the preprint server for biology·2025
Same author

Human milk as a microbial pacemaker.

Cell host & microbe·2025
Same journal

A systematic review of the use of antiretroviral drugs to prevent HIV in neonates and infants.

Journal of the Pediatric Infectious Diseases Society·2026
Same journal

Pediatric inpatient influenza vaccination before, during and after the COVID-19 pandemic.

Journal of the Pediatric Infectious Diseases Society·2026
Same journal

Respiratory bacterial epidemiology at three primary ciliary dyskinesia clinical centers.

Journal of the Pediatric Infectious Diseases Society·2026
Same journal

Epidemiology and Outcomes of Mycobacterium abscessus Infections in immunocompetent children in Singapore.

Journal of the Pediatric Infectious Diseases Society·2026
Same journal

Trimethoprim-Sulfamethoxazole Treatment for Severe Pertussis in Infants Younger Than 2 months During a Macrolide-Resistant Bordetella pertussis Outbreak: A Case Series.

Journal of the Pediatric Infectious Diseases Society·2026
Same journal

Disinvestment in Global Health Threatens Global Security and Children's Health Everywhere.

Journal of the Pediatric Infectious Diseases Society·2026
See all related articles

Related Experiment Video

Updated: Oct 8, 2025

Characterizing Microbiome Dynamics – Flow Cytometry Based Workflows from Pure Cultures to Natural Communities
09:57

Characterizing Microbiome Dynamics – Flow Cytometry Based Workflows from Pure Cultures to Natural Communities

Published on: July 12, 2018

12.1K

Insight Into Host-Microbe Interactions Using Microbial Flow Cytometry Coupled to Next-Generation Sequencing.

Michael A Silverman1,2,3,4, Jamal L Green1,5

  • 1Department of Pediatrics, Division of Infectious Diseases, Children's Hospital of Philadelphia, Philadelphia, Pennsylvania, USA.

Journal of the Pediatric Infectious Diseases Society
|December 24, 2021
PubMed
Summary
This summary is machine-generated.

This review highlights novel microbial flow cytometry coupled to next-generation sequencing (mFLOW-Seq) techniques. These methods integrate antibody responses with microbial identification for advanced infectious disease diagnostics.

Keywords:
antibodymFLOW-Seqmicrobial flow cytometrymicrobiome

More Related Videos

Author Spotlight: Advancing Research in Microbial Autoaggregation Using Imaging Flow Cytometry
05:19

Author Spotlight: Advancing Research in Microbial Autoaggregation Using Imaging Flow Cytometry

Published on: September 29, 2023

939
Integrate Imaging Flow Cytometry and Transcriptomic Profiling to Evaluate Altered Endocytic CD1d Trafficking
09:01

Integrate Imaging Flow Cytometry and Transcriptomic Profiling to Evaluate Altered Endocytic CD1d Trafficking

Published on: October 29, 2018

6.9K

Related Experiment Videos

Last Updated: Oct 8, 2025

Characterizing Microbiome Dynamics – Flow Cytometry Based Workflows from Pure Cultures to Natural Communities
09:57

Characterizing Microbiome Dynamics – Flow Cytometry Based Workflows from Pure Cultures to Natural Communities

Published on: July 12, 2018

12.1K
Author Spotlight: Advancing Research in Microbial Autoaggregation Using Imaging Flow Cytometry
05:19

Author Spotlight: Advancing Research in Microbial Autoaggregation Using Imaging Flow Cytometry

Published on: September 29, 2023

939
Integrate Imaging Flow Cytometry and Transcriptomic Profiling to Evaluate Altered Endocytic CD1d Trafficking
09:01

Integrate Imaging Flow Cytometry and Transcriptomic Profiling to Evaluate Altered Endocytic CD1d Trafficking

Published on: October 29, 2018

6.9K

Area of Science:

  • Microbiology
  • Immunology
  • Genomics

Background:

  • Antibody-based assays are traditional tools for infectious disease diagnostics, leveraging the immune system's response.
  • Next-generation sequencing (NGS) offers direct microbial identification but faces challenges in clinical application.
  • Understanding host-microbial interactions is crucial, as endogenous microbes can act as pathogens.

Purpose of the Study:

  • To review cutting-edge techniques combining antibody responses with microbial analysis.
  • To introduce a unified term, mFLOW-Seq (microbial flow cytometry coupled to NGS), for these integrated approaches.
  • To highlight the potential of these methods in studying host-microbial dynamics.

Main Methods:

  • Coupling antibody detection with flow cytometry for microbe tagging.
  • Utilizing next-generation sequencing (NGS) for microbial identification.
  • Integrating humoral immune responses with direct microbial analysis.

Main Results:

  • These integrated approaches provide a dynamic view of host-microbial relationships.
  • mFLOW-Seq enables simultaneous assessment of immune response and microbial presence.
  • The reviewed techniques offer enhanced sensitivity and specificity in diagnostics.

Conclusions:

  • mFLOW-Seq represents a significant advancement in infectious disease diagnostics.
  • These methods illuminate the complex interplay between the immune system and microbial communities.
  • Future research directions include refining mFLOW-Seq for broader clinical and research applications.