Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

International Consensus Statements on Endoscopic Ultrasound-Guided Biliary Drainage: Results From the 1st JGES International in 2025.

Digestive endoscopy : official journal of the Japan Gastroenterological Endoscopy Society·2026
Same author

A chronic whole cigarette smoke extract model reveals redox-mitochondrial adaptation in human lung epithelial and organoid models.

Experimental & molecular medicine·2026
Same author

Overcoming Extracellular Matrix Barriers: Biomaterial-Assisted microRNA Delivery for Fibrous Musculoskeletal Regeneration.

Aging and disease·2026
Same author

Impact of thermal treatment on genomic DNA integrity and quantification bias in digital PCR.

Methods (San Diego, Calif.)·2026
Same author

Comment on "In-house three dimensional-printed cutting guides improve surgical accuracy in children who underwent resection of malignant bone tumours of lower limb and reconstruction with allograft".

International orthopaedics·2026
Same author

The transformative impact of AI-enabled AlphaFold 3: evolution, current status, and future prospects in structural biology.

Frontiers in artificial intelligence·2026

Related Experiment Video

Updated: Oct 8, 2025

Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification
07:54

Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification

Published on: March 31, 2021

4.8K

Development of SARS-CoV-2 packaged RNA reference material for nucleic acid testing.

Sang-Soo Lee1, Seil Kim1,2,3, Hee Min Yoo1,2

  • 1Bio-Metrology Group, Korea Research Institute of Standards and Science, 267 Gajeong-ro, Yuseong-gu, Daejeon, Korea.

Analytical and Bioanalytical Chemistry
|December 27, 2021
PubMed
Summary

A new lentiviral SARS-CoV-2 reference material (RM) was developed for COVID-19 test quality control. This RM accurately quantifies viral gene copy numbers, enhancing the reliability of molecular diagnostic testing.

Keywords:
Digital PCRLentiviral particleReference material (RM)SARS-CoV-2

More Related Videos

Quantification and Whole Genome Characterization of SARS-CoV-2 RNA in Wastewater and Air Samples
09:26

Quantification and Whole Genome Characterization of SARS-CoV-2 RNA in Wastewater and Air Samples

Published on: June 30, 2023

1.3K
Swabbing the Urban Environment - A Pipeline for Sampling and Detection of SARS-CoV-2 From Environmental Reservoirs
07:13

Swabbing the Urban Environment - A Pipeline for Sampling and Detection of SARS-CoV-2 From Environmental Reservoirs

Published on: April 9, 2021

4.3K

Related Experiment Videos

Last Updated: Oct 8, 2025

Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification
07:54

Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification

Published on: March 31, 2021

4.8K
Quantification and Whole Genome Characterization of SARS-CoV-2 RNA in Wastewater and Air Samples
09:26

Quantification and Whole Genome Characterization of SARS-CoV-2 RNA in Wastewater and Air Samples

Published on: June 30, 2023

1.3K
Swabbing the Urban Environment - A Pipeline for Sampling and Detection of SARS-CoV-2 From Environmental Reservoirs
07:13

Swabbing the Urban Environment - A Pipeline for Sampling and Detection of SARS-CoV-2 From Environmental Reservoirs

Published on: April 9, 2021

4.3K

Area of Science:

  • Virology and Molecular Diagnostics
  • Biotechnology and Reference Materials

Background:

  • The COVID-19 pandemic necessitates reliable SARS-CoV-2 molecular testing.
  • Quality assessment of diagnostic laboratories and molecular products requires robust reference materials (RMs).
  • Existing RMs may not fully replicate the complexities of real-world samples for SARS-CoV-2 detection.

Purpose of the Study:

  • To produce and characterize a lentiviral SARS-CoV-2 reference material (RM).
  • To validate the RM's suitability for quality assessment in SARS-CoV-2 molecular testing.
  • To establish accurate reference values for viral target genes within the RM.

Main Methods:

  • Production of a lentiviral RM containing key SARS-CoV-2 genomic targets (RdRp, N, E, S genes).
  • Quantification using multiple assays on two digital PCR platforms.
  • Assessment of homogeneity and stability via reverse transcription droplet digital PCR (RT-ddPCR) with in-house assays.

Main Results:

  • The lentiviral SARS-CoV-2 RM contains approximately 12 kilobases of the viral genome.
  • Copy number concentrations for target genes range from 1.5 × 10^5 to 2.0 × 10^5 copies/mL.
  • The RM demonstrates good between-bottle homogeneity (4.80-8.23%) and stability at 4°C (1 week) and -70°C (6 months).

Conclusions:

  • The developed lentiviral SARS-CoV-2 RM effectively mimics clinical samples.
  • It provides accurate reference values for absolute viral gene copy numbers.
  • This RM can significantly improve the reliability and quality control of SARS-CoV-2 molecular diagnostic tests.