Biosynthesis of Ag-Pt bimetallic nanoparticles using propolis extract: Antibacterial effects and catalytic activity...

The critical environmental issues of antibiotic resistance and renewable energies supply urge researching materials synthesis and catalyst activity on hydrogen production processes. Aiming to analyse the antibacterial effect of platinum-silver (Ag-Pt) nanoparticles (NPs) and the catalyst effect on NaBH₄ hydrolysis that can be used for hydrogen generation technology, in this work, Ag-Pt NPs were prepared using aqueous propolis extract. Various methods were used for the characterization (Uv-vis Spectroscopy, Transmission Electron Microscopy (TEM), Fourier Transform Infrared Spectroscopy (FTIR), Atomic Force Microscopy (AFM) and X-ray diffraction Spectroscopy (XRD)). The antimicrobial activity of Ag-Pt bimetallic nanoparticles was evaluated in vitro by the microdilution method against Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Klebsiella pneumoniae, Staphylococcus epidermidis, and Serratia marcescens. The results confirmed the antimicrobial activity of bimetallic NPs Ag-Pt concentrations of (25, 50, and 100 μg/ml). A concentration of 100 μg/ml showed low bacterial viability varying between 22.58% and 29.67% for the six tested bacteria. For the catalyst activity on NaBH₄ hydrolysis, the results showed high turnover factor (TOF) and low activation energy of 1208.57 h^-1 and 25.61 kJ/mol, respectively, with high hydrogen yield under low temperature. Synthesized Ag-Pt NPs can have great potential for biological and hydrogen storage applications.