Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Plant Tissue Culture02:57

Plant Tissue Culture

39.0K
Plant tissue culture is widely used in both primary and applied science. Applications range from plant development studies to functional gene studies, crop improvement, commercial micropropagation, virus elimination, and conservation of rare species.
39.0K
Seed Structure and Early Development of the Sporophyte02:33

Seed Structure and Early Development of the Sporophyte

29.6K
Seed structures are composed of a protective seed coat surrounding a plant embryo, and a food store for the developing embryo. The embryo contains the precursor tissues for leaves, stem, and roots. The endosperm and cotyledons—seed leaves—act as the food reserves for the growing embryo.
29.6K
Plant Tissues01:18

Plant Tissues

7.3K
Plants are multicellular eukaryotes with tissue systems made of various cell types that carry out specific functions. Different tissues work together to perform a unique function and form an organ. Organs working together form organ systems. Vascular plants have two distinct organ systems: a shoot system and a root system. The shoot system consists of two portions: the vegetative (non-reproductive) parts of the plant, such as the leaves and the stems, and the reproductive parts of the plant,...
7.3K
Transgenic Plants02:50

Transgenic Plants

7.6K
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
7.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

In Vitro Culture, Genetic Uniformity Assessment, and Biochemical Traits of <i>Plectranthus amboinicus</i> (Lour.) Spreng.

Plants (Basel, Switzerland)·2026
Same author

Rheological Flow Behavior of Six Gelling Agents and Their Relevance for In Vitro Culture Performance of Medicinal Plants.

Gels (Basel, Switzerland)·2026
Same author

Effects of Culture Period and Plant Growth Regulators on In Vitro Biomass Production and Phenolic Compounds in Seven Species of <i>Hypericum</i>.

Plants (Basel, Switzerland)·2025
Same author

The Ligand Binding Domain of the Cell Wall Protein SraP Modulates Macrophage Apoptosis and Inflammatory Responses in <i>Staphylococcus aureus</i> Infections.

Molecules (Basel, Switzerland)·2025
Same author

Red Cabbage Modulates Composition and Co-Occurrence Networks of Gut Microbiota in a Rodent Diet-Induced Obesity Model.

Foods (Basel, Switzerland)·2024
Same author

Chemical Composition of Thyme (<i>Thymus vulgaris</i>) Extracts, Potential Inhibition of SARS-CoV-2 Spike Protein-ACE2 Binding and ACE2 Activity, and Radical Scavenging Capacity.

Journal of agricultural and food chemistry·2023

Related Experiment Video

Updated: Oct 8, 2025

Author Spotlight: Soybean Hairy Root Transformation for the Analysis of Gene Function
07:34

Author Spotlight: Soybean Hairy Root Transformation for the Analysis of Gene Function

Published on: May 5, 2023

4.2K

Soybean Callus-A Potential Source of Tocopherols.

Liliana Mureșan1, Doina Clapa2, Teodor Rusu3

  • 1Diet, Genomics and Immunology Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, MD 20705, USA.

Plants (Basel, Switzerland)
|December 28, 2021
PubMed
Summary

Soybean callus cultures contain significantly higher levels of tocopherols, essential antioxidants, compared to soybean seeds. Herbicides impact both callus production and tocopherol content in vitro.

Keywords:
Glycine maxHPLCin vitro cultureα tocopherolγ tocopherolδ tocopherol

More Related Videos

A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses
09:22

A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses

Published on: January 25, 2018

25.4K
Identification of the Genes Involved in Stomatal Development via Epidermal Phenotype Scoring
05:22

Identification of the Genes Involved in Stomatal Development via Epidermal Phenotype Scoring

Published on: January 20, 2023

1.8K

Related Experiment Videos

Last Updated: Oct 8, 2025

Author Spotlight: Soybean Hairy Root Transformation for the Analysis of Gene Function
07:34

Author Spotlight: Soybean Hairy Root Transformation for the Analysis of Gene Function

Published on: May 5, 2023

4.2K
A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses
09:22

A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses

Published on: January 25, 2018

25.4K
Identification of the Genes Involved in Stomatal Development via Epidermal Phenotype Scoring
05:22

Identification of the Genes Involved in Stomatal Development via Epidermal Phenotype Scoring

Published on: January 20, 2023

1.8K

Area of Science:

  • Plant Biotechnology
  • Agricultural Science
  • Biochemistry

Background:

  • In vitro plant cultures are effective for producing secondary metabolites.
  • Tocopherols (Vitamin E) are vital antioxidants found in soybeans.
  • Understanding tocopherol accumulation in soybean callus is crucial for biotechnological applications.

Purpose of the Study:

  • To quantify and compare tocopherol content (α-, γ-, δ-tocopherol) in soybean callus versus soybean seeds.
  • To investigate the influence of different weed control strategies, including herbicides, on callus production and tocopherol levels.
  • To assess the potential of soybean callus as a source of tocopherols.

Main Methods:

  • Soybean callus was induced using Murashige and Skoog (MS) medium with 6-Benzylaminopurine (BAP) and Thidiazuron (TDZ).
  • Tocopherol content was analyzed in callus and soybean seeds from conventional tillage systems with varying herbicide treatments.
  • Statistical analysis was performed to determine the significance of herbicide effects (p < 0.05).

Main Results:

  • Soybean dry callus exhibited substantially higher concentrations of α-, γ-, and δ-tocopherols compared to soybean seeds.
  • γ-tocopherol was the predominant tocopherol in both soybean callus and seeds.
  • Herbicides significantly influenced in vitro callus production and the accumulation of tocopherols within the callus.

Conclusions:

  • Soybean callus represents a promising source for high-yield tocopherol production.
  • Agricultural practices, particularly herbicide application, critically affect in vitro soybean callus yield and tocopherol content.
  • Further research into optimizing conditions for tocopherol-rich soybean callus is warranted.