Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

11.1K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
11.1K
IR Spectrum Peak Intensity: Amount of IR-Active Bonds00:55

IR Spectrum Peak Intensity: Amount of IR-Active Bonds

743
When infrared radiation is passed through a molecule, absorption occurs if the molecule's vibration leads to a substantial change in its bond dipole moment. Transitions between vibrational energy levels, typically corresponding to infrared frequencies (4000–400 cm−1), allow absorption if the vibration significantly alters the dipole moment, making the molecule infrared active. The molecular bonds have different stretching and bending vibrations, resulting in various peaks with...
743
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

9.3K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
9.3K
Infrared (IR) Spectroscopy: Overview01:09

Infrared (IR) Spectroscopy: Overview

2.6K
When electromagnetic radiation passes through a material, atoms or molecules transition from a lower to a higher energy state by absorbing radiation corresponding to the energy difference between the two states. The absorption of infrared (IR) radiation causes transitions between vibrational energy levels in a molecule. Therefore, IR spectroscopy is a useful analytical tool for determining the molecular structure of molecules.
Different compounds display unique properties due to their...
2.6K
IR Spectrometers01:25

IR Spectrometers

1.6K
There are two main infrared (IR) spectrophotometers: dispersive IR spectrometers and Fourier transform infrared (FTIR) spectrometers. In a dispersive IR spectrometer, a beam of infrared radiation produced by a hot wire is divided into two parallel equal-intensity beams using mirrors. One beam passes through the sample, while another is a reference beam. The beams then move through the monochromator, which separates the radiations into a continuous spectrum of different frequencies. The...
1.6K
IR Spectrum Peak Intensity: Dipole Moment01:20

IR Spectrum Peak Intensity: Dipole Moment

873
The dipole moment of a bond is the product of the partial charge on either atom and the distance between them. Dipole moments influence the efficiency of IR absorption and the peak intensity. When a bond with a dipole moment is placed in an electric field, the direction of the field determines if the bond is compressed or stretched. Electromagnetic radiation consists of an electric field component that rapidly reverses direction. It follows that polar bonds are alternately stretched and...
873

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A customizable, low-cost 3D-printed device for live cell confinement imaging.

Lab on a chip·2026
Same author

Viral Capsid-Membrane Interactions Propel Non-Brownian Movements of Non-enveloped Reoviruses during Entry.

bioRxiv : the preprint server for biology·2026
Same author

Amphiphilic Janus Nanoparticles Synergize with Antibiotics to Restore Susceptibility in Drug-Resistant Gram-Negative Bacteria.

Nano letters·2025
Same author

Harnessing Nanomaterials for Precision Intracellular Sensing.

JACS Au·2025
Same author

Action-based two-dimensional infrared spectroscopy on the horizon.

The Journal of chemical physics·2025
Same author

Targeting the Weak Spot: Preferential Disruption of Bacterial Poles by Janus Nanoparticles.

Nano letters·2024

Related Experiment Video

Updated: Oct 8, 2025

Atomic Force Microscopy of Red-Light Photoreceptors Using PeakForce Quantitative Nanomechanical Property Mapping
14:13

Atomic Force Microscopy of Red-Light Photoreceptors Using PeakForce Quantitative Nanomechanical Property Mapping

Published on: October 24, 2014

11.9K

Dual-Color Peak Force Infrared Microscopy.

Qing Xie1, Jared Wiemann2, Yan Yu2

  • 1Department of Chemistry, Lehigh University, 6 E. Packer Ave., Bethlehem, Pennsylvania 18015, United States.

Analytical Chemistry
|December 28, 2021
PubMed
Summary

Dual-color Peak Force Infrared (PFIR) microscopy enables simultaneous nanoscale infrared imaging at two frequencies. This advancement overcomes limitations of single-frequency PFIR, allowing for precise chemical nanoimaging of materials and biological samples without distortion.

More Related Videos

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

9.1K
High-definition Fourier Transform Infrared FT-IR Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology
11:05

High-definition Fourier Transform Infrared FT-IR Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology

Published on: January 21, 2015

33.4K

Related Experiment Videos

Last Updated: Oct 8, 2025

Atomic Force Microscopy of Red-Light Photoreceptors Using PeakForce Quantitative Nanomechanical Property Mapping
14:13

Atomic Force Microscopy of Red-Light Photoreceptors Using PeakForce Quantitative Nanomechanical Property Mapping

Published on: October 24, 2014

11.9K
Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

9.1K
High-definition Fourier Transform Infrared FT-IR Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology
11:05

High-definition Fourier Transform Infrared FT-IR Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology

Published on: January 21, 2015

33.4K

Area of Science:

  • Spectroscopy
  • Nanotechnology
  • Microscopy

Background:

  • Peak Force Infrared (PFIR) microscopy offers sub-10 nm spatial resolution for infrared imaging.
  • A key limitation of PFIR is the sequential scanning of single infrared frequencies per frame, leading to potential drift and distortion.
  • Simultaneous imaging of multiple frequencies is crucial for comparative nanoscale chemical analysis.

Purpose of the Study:

  • To develop and demonstrate dual-color PFIR microscopy for simultaneous infrared imaging at two frequencies.
  • To overcome the limitations of frame drift and distortion inherent in comparing single-frequency PFIR images.
  • To showcase the application of this technique for chemical nanoimaging of polymers and biological samples.

Main Methods:

  • Implementation of dual-color PFIR microscopy for simultaneous acquisition of infrared data at two distinct frequencies.
  • Utilized atomic force microscopy (AFM) for photothermal mechanical detection.
  • Benchmarked performance and spatial resolution using phase-separated polymers and imaging the cell wall of *Escherichia coli* (*E. coli*).

Main Results:

  • Successfully demonstrated simultaneous nanoscale infrared imaging at two frequencies using dual-color PFIR microscopy.
  • The method effectively bypasses limitations related to frame drift and distortion when comparing images.
  • Successfully mapped the chemical composition of the *E. coli* cell wall, detecting the bacterial outer membrane without labels.

Conclusions:

  • Dual-color PFIR microscopy enables simultaneous, nondestructive chemical nanoimaging of multiple components.
  • This technique significantly enhances the capabilities of PFIR for analyzing complex materials and biological systems.
  • Potential applications include *in situ* dual-channel monitoring of chemical reactions and advanced materials characterization.