Enhancing enzymatic digestibility of bamboo residues using a three-constituent deep eutectic solvent pretreatment

A three-constituent deep eutectic solvent (3c-DES) pretreatment with choline chloride-oxalic acid-ethylene glycol was applied to examine its effectiveness on bamboo residues. The 3c-DES pretreatment can remove 91.09% xylan and significantly improved the 72 h hydrolysis yield of D. sinicus by 6.3 and 1.7 times as compared with the liquid hot water and two-constituent deep eutectic solvent (2c-DES) pretreatment. The introduction of ethylene glycol (EG) into choline chloride (ChCl)/ oxalic acid (OA) decreased the content of surface lignin and the condensation of lignin, which contributed to the increase of hydrophilic nature and cellulose accessibility in substrates. Moreover, higher glucose (85.72%) and xylose (91.05%) yields of 3c-DES pretreated bamboo were achieved with the addition of Tween 80. The 3c-DES system provides an alternative approach for the development of efficient bamboo pretreatment, and had broad space for bamboo biorefinery in southern China.