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An ELISA for anti-thymocyte autoantibody in NZB mice. Comparison with the cytotoxic assay

M H Bocchieri

    Journal of Immunological Methods
    |August 3, 1987
    PubMed

    An ELISA is described for the detection of anti-thymocyte antibody found in the serum of NZB autoimmune mice. This assay avoids the disadvantages of previous assay techniques which require carefully selected complement sources and/or radioactive labelled target cells. Thymocytes from a variety of strains of mice can be used as the antigenic targets, and the plates stored as long as 1 month. The lymphoma line EL4 can be used as the antigenic target in this assay, but it not as sensitive to most serum pools as thymocytes.

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