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Updated: Oct 8, 2025

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Nanocube-Based Fluidic Glycan Array.

Hung-Jen Wu1, Akshi Singla2, Joshua D Weatherston2

  • 1Department of Chemical Engineering, Texas A&M University, College Station, TX, USA. hjwu@tamu.edu.

Methods in Molecular Biology (Clifton, N.J.)
|January 1, 2022
PubMed
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This study introduces a plasmonic nanocube sensor for detecting lectin binding kinetics in cell membrane environments. The assay uses standard lab equipment, offering a new method for studying cell surface interactions.

Area of Science:

  • Biophysics
  • Materials Science
  • Analytical Chemistry

Background:

  • Cell membrane fluidity allows surface glycan diffusion, influencing lectin binding.
  • Weakly binding glycans can participate in dynamic lectin-glycan interactions.

Purpose of the Study:

  • To present a plasmonic nanocube sensor for detecting lectin binding kinetics.
  • To provide a method for studying cell surface interactions in a membrane-mimicking environment.

Main Methods:

  • Fabrication of plasmonic nanocube sensors.
  • Sensor calibration and data processing protocols.
  • Assay for detecting lectin-glycan interactions using standard spectrometers.

Main Results:

  • The sensor enables detection of lectin binding kinetics.
Keywords:
Fluidic membraneGlycan arrayHetero-multivalencyLectinLocalized surface plasmon resonanceMultivalent bindingNanocube sensorReduction of dimensionalitySupported lipid bilayer

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  • The assay is compatible with standard laboratory equipment like microplate readers.
  • Detailed protocols and troubleshooting guide are provided.

    • Conclusions:

    • Plasmonic nanocube sensors offer a viable tool for studying cell surface glycan-lectin interactions.
    • The described assay simplifies the kinetic analysis of these binding events.
    • This technology facilitates research in cell membrane dynamics and interactions.