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A new storage solution for packed red blood cells (PRBCs) significantly reduces the storage lesion and post-resuscitation inflammation. This innovation may improve PRBC storage and reduce transfusion-related harm.

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Area of Science:

  • Hematology
  • Transfusion Medicine
  • Biomaterials

Background:

  • Massive transfusion of aged packed red blood cells (PRBCs) is linked to increased patient morbidity and mortality.
  • The "storage lesion" describes biochemical and structural changes in PRBCs during storage, negatively impacting their function.
  • A novel storage solution was developed to increase viscosity and potentially mitigate PRBC storage lesion.

Purpose of the Study:

  • To evaluate the efficacy of a novel storage solution (EAS-1587) in blunting the storage lesion in PRBCs.
  • To assess the impact of PRBCs stored in EAS-1587 on the inflammatory response following resuscitation.
  • To compare the effects of EAS-1587 with standard AS-3 storage solution.

Main Methods:

  • PRBCs from mice and humans were stored for 14 or 42 days in AS-3 or EAS-1587.
  • Analysis included microvesicles, cell-free hemoglobin, phosphatidylserine, band-3 protein, glucose utilization, and osmotic fragility.
  • Mice underwent hemorrhage and resuscitation with PRBCs stored in either solution, with serum analyzed for inflammatory markers.

Main Results:

  • Murine PRBCs in EAS-1587 showed reduced microvesicles, cell-free hemoglobin, and phosphatidylserine expression, with preserved band-3 and improved glucose utilization and osmotic resistance.
  • Resuscitation with EAS-1587 stored PRBCs in mice led to lower serum proinflammatory cytokines.
  • Human PRBCs in EAS-1587 demonstrated reduced microvesicles, cell-free hemoglobin, and increased glucose utilization.

Conclusions:

  • Storage of PRBCs in the novel EAS-1587 solution effectively mitigated key aspects of the storage lesion.
  • The modified storage solution reduced the inflammatory response associated with resuscitation after hemorrhage.
  • This novel storage solution holds promise for improving PRBC quality and reducing harm in massive transfusion scenarios.