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Development of an Online Tool for Pasteurella multocida Genotyping and Genotypes of Pasteurella multocida From
Zhong Peng1, Junyang Liu2, Wan Liang1,3
1State Key Laboratory of Agricultural Microbiology, The Cooperative Innovation Center for Sustainable Pig Production, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.
Abstract:
Pasteurella multocida is a versatile zoonotic pathogen. Multiple systems have been applied to type P. multocida from different diseases in different hosts. Recently, we found that assigning P. multocida strains by combining their capsular, lipopolysaccharide, and MLST genotypes (marked as capsular: lipopolysaccharide: MLST genotype) could help address the biological characteristics of P. multocida circulation in different hosts. However, there is still lack of a rapid and efficient tool to diagnose P. multocida according to this system. Here, we developed an intelligent genotyping platform PmGT for P. multocida strains according to their whole genome sequences using the web 2.0 technologies. By using PmGT, we determined capsular genotypes, LPS genotypes, and MLST genotypes as well as the main virulence factor genes (VFGs) of P. multocida isolates from different host species based on their whole genome sequences published on NCBI. The results revealed a closer association between the genotypes and pasteurellosis rather than between genotypes and host species. With the advent of high-quality, inexpensive DNA sequencing, PmGT represents a more efficient tool for P. multocida diagnosis in both epidemiological studies and clinical settings.
Insights
A new tool, Pasteurella multocida Genotyping Tool (PmGT), rapidly determines Pasteurella multocida genotypes from whole genome sequences. This aids in diagnosing pasteurellosis by linking genotypes to disease, not just host species.
Area of Science:
- Veterinary Microbiology
- Genomics
- Bioinformatics
Background:
- Pasteurella multocida is a zoonotic pathogen with diverse disease associations.
- Current typing systems for P. multocida are insufficient for efficient diagnosis.
- Combining capsular, lipopolysaccharide (LPS), and MLST genotypes offers insights into P. multocida circulation.
Purpose of the Study:
- To develop a rapid and efficient tool for P. multocida genotyping based on whole genome sequences.
- To integrate capsular, LPS, and MLST genotyping with virulence factor gene identification.
- To analyze the association between P. multocida genotypes and disease manifestations.
Main Methods:
- Developed the intelligent genotyping platform PmGT using web 2.0 technologies.
- Utilized whole genome sequences of P. multocida isolates from NCBI.
- Determined capsular, LPS, MLST genotypes, and main virulence factor genes (VFGs) using PmGT.
Main Results:
- PmGT successfully determined genotypes and VFGs for P. multocida isolates.
- Genotypes showed a stronger association with pasteurellosis than with host species.
- The platform facilitates diagnosis in epidemiological and clinical settings.
Conclusions:
- PmGT provides an efficient method for P. multocida genotyping and diagnosis.
- Genotype-disease associations are more significant than genotype-host associations.
- This tool supports Pasteurella multocida surveillance and clinical management.
