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A Method for the Measurement of Salivary Gland Function in Mice
Published on: January 25, 2018
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Protocol for parotidectomy and saliva analysis in mice
Khaled Zubeidat1, Yasmin Saba1, Or Barel1
1Institute of Biomedical and Oral Research, Faculty of Dental Medicine, Hebrew University, Jerusalem, Israel.
STAR Protocols
|January 3, 2022
Summary
This study presents a protocol for parotidectomy and saliva collection in mice. These methods allow for the study of salivary gland development and oral mucosal immunity in young and adult animals.
Area of Science:
- Immunology
- Developmental Biology
- Surgical Techniques
Background:
- Murine parotid salivary glands are crucial for developing oral mucosal immunity.
- These glands play a role in both early and adult oral health.
- Existing protocols may not be suitable for collecting saliva from young mice.
Purpose of the Study:
- To detail a protocol for parotidectomy (surgical removal) in mice.
- To describe a modified saliva collection method for mice, including young ones.
- To enable the study of physiological and immunological salivary functions.
Main Methods:
- Surgical removal of parotid glands in mice.
- Saliva collection from mice using a modified technique.
- Measurement of physiological and immunological salivary functions.
Main Results:
- A comprehensive protocol for parotidectomy in mice is established.
- A modified saliva collection technique is presented, adaptable for young mice.
- The protocol facilitates the investigation of salivary gland functions.
Conclusions:
- The described protocol provides a reliable method for parotidectomy and saliva collection in mice.
- This technique is valuable for research into oral mucosal immunity and salivary gland function.
- The modified saliva collection is particularly useful for studying developmental aspects of salivary function.
