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Dark fermentative biohydrogen production from vinicultural biomass without exogenous inoculum in a semi-batch
Zineb Guellout1, Emilie Francois-Lopez2, Yacine Benguerba3
1Département de Génie des Procédés, Faculté de Technologie, Université Chahid Mostafa BENBOULAID Batna 2, Algeria; Université de Strasbourg, CNRS, IPHC UMR 7178, Laboratoire de Reconnaissance et Procédés de Séparation Moléculaire (RePSeM), ECPM 25 rue Becquerel, F-67000, Strasbourg, France.
Abstract:
This work employed a unique kind of vinicultural biomass (grape residues) to generate fermentative hydrogen. This form of biomass serves two purposes (contains substrate and inoculum). Four mathematical model methods were established; these models were used to represent the fluctuation of hydrogen generation and other fermentation products (organic acids, alcohols), the consumption of substrates included in biomass, and bacterial growth. One of these models was verified using experimental data and used to represent all of the metabolic pathways of bacteria contained in the medium and the interaction between products and substrates. The optimal biomass load, 60 g COD (Chemical Oxygen Demand)/L with a concentration of 0.22 mol of hexose and 0.0444 mol of tartrate offers the best hydrogen yield.
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