Related Experiment Video
Updated: Oct 8, 2025

Nanopore DNA Sequencing for Metagenomic Soil Analysis
Published on: December 14, 2017
Fast nanopore sequencing data analysis with SLOW5
Hasindu Gamaarachchi1,2, Hiruna Samarakoon3,4, Sasha P Jenner3
1Kinghorn Centre for Clinical Genomics, Garvan Institute of Medical Research, Sydney, New South Wales, Australia. hasindu@garvan.org.au.
Abstract:
Nanopore sequencing depends on the FAST5 file format, which does not allow efficient parallel analysis. Here we introduce SLOW5, an alternative format engineered for efficient parallelization and acceleration of nanopore data analysis. Using the example of DNA methylation profiling of a human genome, analysis runtime is reduced from more than two weeks to approximately 10.5 h on a typical high-performance computer. SLOW5 is approximately 25% smaller than FAST5 and delivers consistent improvements on different computer architectures.
Related Concept Videos
Next-generation Sequencing
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
Sanger Sequencing

