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Labeling DNA Probes03:31

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems
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Hypoxia-Activated Fluorescent Probe Based on Self-Immolative Block Copolymer.

Kuanchun Shao1, Wenlong Zhang1, Jiajia Shen1

  • 1Department of Chemical Engineering, Key Laboratory of Advanced Materials (MOE), Tsinghua University, Beijing, 100084, China.

Macromolecular Bioscience
|January 4, 2022
PubMed
Summary

This study developed a hypoxia-activated fluorescent probe for tumor imaging. The probe uses a self-immolative polymer that releases fluorescent signals in the low-oxygen tumor environment, enabling effective cancer detection.

Keywords:
azobenzene, fluorescent probeshypoxia responsiveself-immolative polymers

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Area of Science:

  • Polymer Chemistry
  • Biomedical Imaging
  • Chemical Biology

Background:

  • Tumor microenvironments often exhibit hypoxia.
  • Developing targeted imaging probes for tumors is crucial for early diagnosis and treatment.

Purpose of the Study:

  • To design and synthesize a hypoxia-activated fluorescent probe for tumor imaging.
  • To investigate the self-immolative polymer's response to hypoxic conditions.

Main Methods:

  • Utilized a self-immolative block copolymer with an azobenzene linkage.
  • Explored the reduction of azobenzene by azoreductase in hypoxic tumor cells.
  • Observed the polymer disassembly and subsequent fluorescence emission.

Main Results:

  • The water-soluble polymer showed no inherent fluorescence.
  • Hypoxia triggered azobenzene reduction and polymer disassembly.
  • Released building blocks emitted strong fluorescence, indicating successful probe activation.

Conclusions:

  • The developed probe effectively responds to tumor hypoxia.
  • This hypoxia-activated fluorescent probe shows significant potential for tumor imaging applications.