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Related Experiment Video

Updated: Oct 7, 2025

A Mouse Model to Evaluate the Long-Term Structural and Functional Outcomes after the Reversal of Prolonged Unilateral Ureteric Obstruction

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Analyze Mouse Knockout Models of UPR Pathway Elements.

Chao Zheng¹, Cheng Wang², Qiang Jie³

¹Institute of Orthopedic Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an, China.

Methods in Molecular Biology (Clifton, N.J.)

|January 5, 2022

Summary

This summary is machine-generated.

Aberrant unfolded protein response (UPR) signaling can cause defective skeletal development. This chapter details methods for studying UPR-induced skeletal disorders in genetically modified mice, focusing on skeletal morphology and protein secretion.

Keywords:

Chondrocyte Immunostaining assay Matrix secretion Mouse genotyping Skeletal preparation UPR

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Area of Science:

Molecular Biology
Genetics
Developmental Biology

Background:

Genetic studies in humans and mice link aberrant unfolded protein response (UPR) signaling to defective skeletal development.
Investigating skeletal phenotypes in UPR-related mouse models requires visualizing morphological alterations and assessing protein secretion and UPR activation.

Purpose of the Study:

To introduce key techniques for studying UPR-induced skeletal disorders in genetically modified mice.
To provide detailed protocols for mouse genotyping, bone tissue grossing, whole-mount skeletal staining, and immunostaining assays.

Main Methods:

Mouse genotyping for genetic modification identification.
Bone tissue grossing and preparation for analysis.
Whole-mount skeletal staining for morphological assessment.
Immunostaining assays to evaluate matrix secretion and UPR activation.

Main Results:

The chapter outlines established laboratory techniques for analyzing skeletal phenotypes.
Methods cover the initial steps from genetic validation to detailed tissue analysis.

Conclusions:

The described methods are crucial for investigating the role of UPR signaling in skeletal disorders.
This chapter serves as a practical guide for researchers studying UPR-related skeletal phenotypes in mouse models.