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Updated: Oct 7, 2025
Culture Methods to Determine the Limit of Detection and Survival in Transport Media of Campylobacter Jejuni in Human Fecal Specimens
Published on: March 10, 2020
Clinical Utility of BDTM mCCDA ClearHT Agar Medium for Campylobacter jejuni
Mitsunori Kaneda1, Kumiko Takesue2, Keisuke Mori2
1Department of Clinical Laboratory, Medical Diagnostic Center of URAWA Medical Association, 6‒4‒18 Tokiwa, Urawa-ku, Saitama 338‒0011, Japan. Department of Natural Sciences, Graduate School of Integrative Science and Engineering, Tokyo City University, 1‒28‒1 Tamatsutsumi, Setagaya-ku, Tokyo 158‒8557, Japan.
Abstract:
Campylobacter spp. has been the leading cause of bacterial food poisoning in Japan since 2000. The predominant Campylobacter spp. testing method employs selective medium to isolate Campylobacter spp. In the present study, we evaluated the Campylobacter-isolating capacity and clinical utility of BDTM mCCDA Clear-HT, an agar medium containing a chromogenic substrate, on 230 diarrhea stool samples. After 48 hours incubation, 50 samples (21.7%) were positive with BDTM mCCDA Clear-HT, while 61 samples (26.5%) were positive using modified Skirrow agar medium. In this study, BDTM mCCDA Clear-HT had a lower detection rate of Campylobacter jejuni more than Vitalmedia modified Skirrow agar medium.
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