Emergence and patterning dynamics of mouse-definitive endoderm
Maayan Pour1, Abhishek Sampath Kumar2, Naama Farag1
1School of Neurobiology, Biochemistry and Biophysics, Department of Biochemistry and Molecular Biology, Tel Aviv University, Tel Aviv 6997801, Israel.
Iscience
|January 6, 2022
Summary
Definitive endoderm (DE) emerges from progenitors in a precise temporal window. This study reveals DE onset dynamics using a dual reporter, showing spatial stochasticity and self-sorting mechanisms in embryonic development.
Area of Science:
- Developmental Biology
- Stem Cell Biology
- Genetics
Background:
- Definitive endoderm (DE) formation is crucial for germ layer development.
- Understanding DE commitment onset is difficult due to its rapid spatiotemporal occurrence in embryos.
Purpose of the Study:
- To investigate the dynamics of definitive endoderm (DE) onset and patterning.
- To elucidate the mechanisms governing DE cell emergence from mesendoderm progenitors.
Main Methods:
- Utilized a dual Bra/Sox17 reporter cell line for in vivo and in vitro studies.
- Analyzed DE onset dynamics in 2D and 3D culture models.
- Investigated cell sorting and gene expression changes during DE commitment.
Main Results:
- Sox17 expression initiates in isolated cells within a specific temporal window in vivo.
- DE cells emerge stochastically but are patterned by self-sorting of Sox17+ cells.
- A Bra-high subpopulation commits to Sox17+ fate independently of Wnt signaling.
Conclusions:
- DE onset follows basic rules of temporal regulation and spatial patterning.
- Self-sorting of Sox17+ cells contributes to DE arrangement but is not essential for differentiation.
- Commonalities and differences between in vivo and in vitro systems offer insights into DE development.
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