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Fourier spotting: a novel setup for single-color reflectometry.

Johannes Siegel1, Marcel Berner2, Juergen H Werner2

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This study introduces a novel single-color reflectometry setup for label-free bioanalysis. The system accurately detects refractive index changes and analyzes binding kinetics, demonstrating its suitability for reliable biosensing applications.

Keywords:
BiosensorFourier spottingLight modulationSingle-color reflectometry

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Area of Science:

  • Optical biosensing
  • Label-free bioanalysis
  • Surface plasmon resonance

Background:

  • Single-color reflectometry is a sensitive optical biosensor technique.
  • Existing methods can suffer from baseline offset and drift.
  • Label-free detection is crucial for real-time bioanalysis.

Purpose of the Study:

  • To present a novel single-color reflectometry setup using differential Fourier spotting.
  • To demonstrate its capability for label-free detection and kinetic analysis.
  • To reduce baseline offset and drift in optical biosensing.

Main Methods:

  • Utilized patented micro-mirror technology for signal encoding.
  • Employed a single photodiode for simultaneous analyte and reference channel detection.
  • Implemented a 180° phase shift for baseline offset reduction.

Main Results:

  • Achieved detection of refractive index changes as low as Δn < 0.01.
  • Validated heterogeneous binding interaction detection (antibody/antigen).
  • Determined binding kinetics with an affinity constant KA = 1.59 × 10-7 M-1.

Conclusions:

  • The differential Fourier spotting setup is effective for reliable bioanalysis.
  • The system offers robust and sensitive label-free detection.
  • This technology advances optical biosensor capabilities.