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Updated: Oct 7, 2025

3D Multicolor DNA FISH Tool to Study Nuclear Architecture in Human Primary Cells
Published on: January 25, 2020
RNA gradients: Shapers of 3D genome architecture
Britta A M Bouwman1, Nicola Crosetto1, Magda Bienko1
1Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, 17165, Sweden; Science for Life Laboratory, Solna, 17165, Sweden.
Abstract:
A growing body of evidence points to a role of nuclear RNAs (nucRNAs) in shaping the three-dimensional (3D) architecture of the genome within the nucleus of a eukaryotic cell. nucRNAs are non-homogeneously distributed within the nucleus where they can form global and local gradients that might contribute to instructing the formation and coordinating the function of different types of 3D genome structures. In this article, we highlight the available literature supporting a role of nucRNAs as 3D genome shapers and propose that nucRNA gradients are key mediators of genome structure and function.
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