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Optimizing Extracellular Vesicle Delivery Using a Core-Sheath 3D-Bioprinted Scaffold for Chronic Wound Management
Published on: February 28, 2025
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Nanofiber/hydrogel core-shell scaffolds with three-dimensional multilayer patterned structure for accelerating
Jiankai Li1, Tianshuai Zhang1, Mingmang Pan2
1College of Chemical and Materials Sciences, Shanghai Normal University, No. 100 Guilin Road, Shanghai, 200234, People's Republic of China.
Journal of Nanobiotechnology
|January 9, 2022
Summary
This study introduces a novel 3D nanofiber/hydrogel scaffold that significantly enhances diabetic wound healing by promoting cell activity and blood vessel formation. The scaffold improves wound environment and accelerates tissue regeneration.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Wound Healing Research
Background:
- Impaired angiogenesis is a primary cause of non-healing diabetic wounds.
- Current treatments often fall short in addressing the complex healing environment of diabetic ulcers.
Purpose of the Study:
- To develop and evaluate a novel three-dimensional (3D) nanofiber/hydrogel core-shell scaffold for promoting diabetic wound healing.
- To investigate the scaffold's ability to enhance angiogenesis and facilitate tissue regeneration in diabetic wounds.
Main Methods:
- Fabrication of a 3D multilayer patterned core-shell scaffold (3D-PT-P/GM) using Gelatin methacryloyl (GelMA) hydrogel and Poly (D, L-lactic acid) (PDLLA).
- Characterization of scaffold properties including structure, porosity, and permeability.
- In vitro assessment of cell adhesion, proliferation, migration, and gene expression.
- In vivo evaluation of wound healing, angiogenesis, and tissue formation in a diabetic wound model.
Main Results:
- The 3D-PT-P/GM scaffolds exhibited enhanced porosity, water retention, and permeability compared to 2D scaffolds.
- In vitro studies demonstrated significantly improved cell adhesion, proliferation, infiltration, migration, and upregulation of angiogenesis-related genes.
- In vivo experiments showed accelerated diabetic wound healing with enhanced capillary network formation, granulation tissue development, and collagen deposition.
Conclusions:
- The developed 3D-PT-P/GM scaffolds effectively promote diabetic wound healing by improving the wound microenvironment and stimulating angiogenesis.
- These scaffolds represent a promising new strategy for the treatment of chronic wounds, particularly in diabetic patients.
