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Apostichopus japonicus matrix metalloproteinase-16 might act as a pattern recognition receptor

Jingwei Jiang1, Shan Gao1, Zhong Chen1

  • 1Liaoning Key Laboratory of Marine Fishery Molecular Biology, Liaoning Key Lab of Germplasm Improvement and Fine Seed Breeding of Marine Aquatic Animals, Liaoning Ocean and Fisheries Science Research Institute, Dalian, Liaoning, 116023, PR China.

Fish & Shellfish Immunology
|January 9, 2022
PubMed

Insights

Matrix metalloproteinase-16 (AjMMP-16) in sea cucumbers acts as a pattern recognition receptor. This protein binds to microbial molecules and bacteria, potentially initiating innate immune responses via the MyD88-dependent Toll-like receptor pathway.

Area of Science:

  • Marine biology
  • Immunology
  • Biochemistry

Background:

  • Matrix metalloproteinases (MMPs) are crucial for physiological processes and immune responses.
  • The specific role of MMPs in innate immunity, particularly in invertebrates, requires further elucidation.

Purpose of the Study:

  • To investigate the function of MMP-16 from the sea cucumber Apostichopus japonicus (AjMMP-16) in innate immunity.
  • To analyze the microbial polysaccharide-induced transcriptional expression and binding capabilities of AjMMP-16.

Main Methods:

  • Quantitative real-time PCR for gene expression analysis.
  • Pearson's correlation test to assess gene relationships.
  • Prokaryotic recombinant expression of AjMMP-16.
  • ELISA and indirect immunofluorescence for binding assays.

Main Results:

  • AjMMP-16 expression was significantly induced by various microbial polysaccharides (PGN, LPS, mannan, β-1,3-glucan).
  • AjMMP-16 showed a strong correlation with MyD88 in gene expression.
  • Recombinant AjMMP-16 demonstrated binding to multiple microbial polysaccharides and bacterial species.

Conclusions:

  • AjMMP-16 functions as a pattern recognition receptor in innate immunity.
  • It plays a role in initiating the MyD88-dependent Toll-like receptor signaling pathway.
Keywords:
Apostichopus japonicusImmune signal transductionMatrix metalloproteinase-16Pathogen recognitionRecombinant expressionTranscriptional expression

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