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Transcriptome analysis of acute high temperature-responsive genes and pathways in Palaemon gravieri
Wenjun Shi1, Runhao Hu2, Pan Wang2
1Institute of Oceanology & Marine Fisheries, Jiangsu, Nantong, China; Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, China.
Summary
High temperatures harm shrimp farming. This study shows acute heat stress reduces shrimp metabolism but boosts immunity as a survival response, offering insights for aquaculture.
Area of Science:
- Aquaculture
- Marine Biology
- Environmental Physiology
Background:
- Temperature is a critical factor influencing aquatic animal health, survival, and development in aquaculture.
- Palaemon gravieri, an economically important shrimp, exhibits poor tolerance to high temperatures, hindering large-scale farming.
- Limited research exists on the effects of acute high-temperature stress on P. gravieri.
Purpose of the Study:
- To investigate the physiological and molecular responses of Palaemon gravieri to acute high-temperature stress.
- To identify key genes and pathways involved in shrimp's adaptation to thermal stress.
Main Methods:
- P. gravieri were subjected to a simulated acute high-temperature stress (15°C to 30°C over 3 hours, then 12 hours at 30°C).
- Hepatopancreas tissues were collected at multiple time points for transcriptome analysis.
- Differential gene expression analysis was performed on selected time points (G0, G1, G4).
Main Results:
- Transcriptome analysis revealed 7,744 differentially expressed genes (DEGs) among 18,308 annotated unigenes.
- DEGs were primarily associated with metabolism, immunity (e.g., HSP70, CASP7), and stress responses (e.g., UGT, GST, HSP60/90).
- Acute high temperature significantly decreased metabolic capacity while enhancing immune responses, suggesting a compensatory mechanism.
Conclusions:
- Acute high temperature induces significant metabolic and immune system changes in P. gravieri.
- The observed immune enhancement may represent an emergency metabolic compensation strategy for stress resistance.
- Findings provide crucial insights into the molecular mechanisms underlying P. gravieri's response to thermal stress, aiding aquaculture management.
