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KV7.1 channel blockade inhibits neonatal renal autoregulation triggered by a step decrease in arterial pressure.

Dieniffer Peixoto-Neves1, Praghalathan Kanthakumar1, Jeremiah M Afolabi1

  • 1Department of Physiology, University of Tennessee Health Science Center, Memphis, Tennessee.

American Journal of Physiology. Renal Physiology
|January 10, 2022
PubMed
Summary
This summary is machine-generated.

Neonatal pig renal microvessels express KV7.1 channels, which do not control basal tone but are crucial for renal autoregulation following a drop in blood pressure.

Keywords:
KCNQKV7.1neonatesrenal autoregulationsmooth muscle cells

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Area of Science:

  • Physiology
  • Cardiovascular Research
  • Renal Physiology

Background:

  • KV7 channels (encoded by KCNQ genes) influence vascular responses, but their role in the neonatal kidney is unknown.
  • Previous studies show postnatal changes in KV7 channel expression in rodent arteries.

Purpose of the Study:

  • To investigate the expression and function of KCNQ isoforms in neonatal pig renal microvessels.
  • To determine the role of KV7.1 in renal vascular tone and autoregulation in neonatal pigs.

Main Methods:

  • Detected KCNQ gene expression in neonatal pig renal microvessels.
  • Activated KV7.1 channels in smooth muscle cells and assessed effects using a selective blocker (HMR1556).
  • Measured effects of HMR1556 on isolated microvessels, arterial pressure, renal blood flow, and renal vascular resistance during autoregulation challenges.

Main Results:

  • Only KCNQ1 (KV7.1) was detected in neonatal pig renal microvessels, present from gestation day 50 to postnatal day 21.
  • KV7.1 activation produced currents in smooth muscle cells, inhibited by HMR1556.
  • HMR1556 did not affect basal renal vascular tone but inhibited renal autoregulation following a 20 mmHg reduction in mean arterial pressure.

Conclusions:

  • KCNQ isoform expression in porcine renal microvessels is maturation-dependent.
  • KV7.1 is functionally expressed in neonatal pig renal vascular smooth muscle cells.
  • SMC KV7.1 plays a role in neonatal renal autoregulation, not basal vascular tone.