Related Experiment Video
Updated: Oct 7, 2025
08:21
A Modified Two Kidney One Clip Mouse Model of Renin Regulation in Renal Artery Stenosis
Published on: October 26, 2020
5.1K
KV7.1 channel blockade inhibits neonatal renal autoregulation triggered by a step decrease in arterial pressure
Dieniffer Peixoto-Neves1, Praghalathan Kanthakumar1, Jeremiah M Afolabi1
1Department of Physiology, University of Tennessee Health Science Center, Memphis, Tennessee.
American Journal of Physiology. Renal Physiology
|January 10, 2022
Summary
Neonatal pig renal microvessels express KV7.1 channels, which do not control basal tone but are crucial for renal autoregulation following a drop in blood pressure.
Area of Science:
- Physiology
- Cardiovascular Research
- Renal Physiology
Background:
- KV7 channels (encoded by KCNQ genes) influence vascular responses, but their role in the neonatal kidney is unknown.
- Previous studies show postnatal changes in KV7 channel expression in rodent arteries.
Purpose of the Study:
- To investigate the expression and function of KCNQ isoforms in neonatal pig renal microvessels.
- To determine the role of KV7.1 in renal vascular tone and autoregulation in neonatal pigs.
Main Methods:
- Detected KCNQ gene expression in neonatal pig renal microvessels.
- Activated KV7.1 channels in smooth muscle cells and assessed effects using a selective blocker (HMR1556).
- Measured effects of HMR1556 on isolated microvessels, arterial pressure, renal blood flow, and renal vascular resistance during autoregulation challenges.
Main Results:
- Only KCNQ1 (KV7.1) was detected in neonatal pig renal microvessels, present from gestation day 50 to postnatal day 21.
- KV7.1 activation produced currents in smooth muscle cells, inhibited by HMR1556.
- HMR1556 did not affect basal renal vascular tone but inhibited renal autoregulation following a 20 mmHg reduction in mean arterial pressure.
Conclusions:
- KCNQ isoform expression in porcine renal microvessels is maturation-dependent.
- KV7.1 is functionally expressed in neonatal pig renal vascular smooth muscle cells.
- SMC KV7.1 plays a role in neonatal renal autoregulation, not basal vascular tone.
More Related Videos
Related Concept Videos
01:29Renal Regulation of Acid-Base Balance
802
Metabolic reactions in the body produce nonvolatile acids, such as sulfuric acid, which generate an acid load of approximately 1 mEq of H+ per kilogram of body weight daily. Excreting H+ in the urine is essential to balance this acid load.
In the kidneys, cells within the proximal convoluted tubules (PCT) and the collecting ducts secrete hydrogen ions (H+) into the tubular fluid. Specifically, in the PCT, Na+/H+ antiporters secrete H+ while reabsorbing Na+.
However, the intercalated cells in...
In the kidneys, cells within the proximal convoluted tubules (PCT) and the collecting ducts secrete hydrogen ions (H+) into the tubular fluid. Specifically, in the PCT, Na+/H+ antiporters secrete H+ while reabsorbing Na+.
However, the intercalated cells in...
802
01:28Antihypertensive Drugs: Potassium-Sparing Diuretics
868
Liddle syndrome is a genetically inherited form of hypertension characterized by the overactivity of epithelial sodium channels in the nephron, the functional unit of the kidney. This heightened activity leads to increased sodium reabsorption and excessive excretion of potassium. To counteract this, potassium-sparing diuretics such as amiloride are used. They function by blocking these sodium channels, thereby reducing the influx of sodium into the epithelial cells and minimizing the loss of...
868
01:18Hypertension and Regulation of Blood Pressure
3.3K
Hypertension, the most common cardiovascular disease, is diagnosed through repeated measurements of elevated blood pressure. Its risks, including damage to the kidney, heart, and brain, are directly proportional to blood pressure levels. Starting from 115/75 mm Hg, the risk of cardiovascular disease doubles with each increment of 20/10 mm Hg. The diagnosis relies on blood pressure measurements, not on patient symptoms, as hypertension is often asymptomatic until end-organ damage is imminent or...
3.3K
01:29Acute Kidney Injury II: Pathophysiology
147
Acute kidney injury (AKI) causes are categorized into three primary categories based on the location of the injury: prerenal, intrarenal (or intrinsic), and postrenal causes. This classification guides clinical management and illustrates how different pathways can impair kidney function.Etiology and Pathophysiology of Acute Kidney Injury1. Prerenal causesEtiology: Prerenal Acute Kidney Injury, the most common type, occurs when reduced blood flow to the kidneys decreases filtration capacity...
147
01:25Antihypertensive Drugs: Direct Renin Inhibitors
883
The renin-angiotensin-aldosterone system (RAAS) is an intricate physiological pathway involving numerous enzymes and hormones, including renin, angiotensin-converting enzyme (ACE), angiotensin I and II, and aldosterone. Imbalances within this system increase the production of angiotensin II and aldosterone. Increased angiotensin II levels promote vasoconstriction and blood pressure elevation. Concurrently, higher aldosterone levels stimulate sodium and water reabsorption in the kidneys,...
883
01:17Autoregulation of Blood Flow
3.6K
Autoregulation mechanisms are characterized by their inherent capacity for self-regulation without necessitating specific nervous stimulation or endocrine control. These mechanisms facilitate the adjustment of blood flow and, therefore, perfusion specific to each tissue region. This self-regulation encompasses chemical signals and myogenic controls.
Chemical Signaling in Autoregulation
Chemical signaling operates at the precapillary sphincter level, inciting either contraction or relaxation....
Chemical Signaling in Autoregulation
Chemical signaling operates at the precapillary sphincter level, inciting either contraction or relaxation....
3.6K