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We developed a mobile acousto-optic imaging system for mapping light distribution in scattering samples. Precise synchronization of lasers and ultrasound ensures high-quality, accurate imaging even with parameter instabilities.

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Area of Science:

  • Biomedical Optics
  • Acousto-Optic Imaging
  • Laser-Based Sensing

Background:

  • Optical imaging of scattering media is challenging due to light diffusion.
  • Reflection-mode imaging requires specialized systems for subsurface visualization.
  • Existing systems may lack mobility or precise control over laser-ultrasound synchronization.

Purpose of the Study:

  • To develop a compact, mobile acousto-optic imaging system for reflection-mode operation.
  • To address challenges of laser parameter instability and synchronization in acousto-optic imaging.
  • To map light fluence rate distribution in optically scattering samples with single-sided access.

Main Methods:

  • Utilized a reflection-mode acousto-optic imaging system with dual lasers (532 nm and 1064 nm) and nanosecond pulses.
  • Employed an acousto-optic probe with an ultrasound linear array, optical fiber, and camera for single-sided sample access.
  • Developed an electronic feedback circuit and microcontroller system for precise ultrasound and laser pulse synchronization.

Main Results:

  • Demonstrated successful mapping of light fluence rate distribution in scattering phantoms.
  • Achieved high imaging quality and accuracy through a novel synchronization algorithm and control system.
  • Showcased improvements in signal-to-noise ratio by adjusting region of interest and laser pulse parameters.

Conclusions:

  • The developed acousto-optic imaging system offers a mobile and accurate solution for subsurface light distribution mapping.
  • Precise synchronization is critical for mitigating laser instabilities and ensuring reliable imaging performance.
  • The system's design and control algorithms enable robust imaging of scattering samples with single-sided access.