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T-lymphocyte colony formation of murine thymocytes in agar contained in glass capillaries
H P Matthiessen1, C Echarti, J Gerber
1Institut für Pharmazie der Freien Universität Berlin, Germany.
Blut
|December 1, 1987
Abstract:
Optimal growth conditions are presented for a new colony test with mouse thymocytes in agar contained in glass capillaries. The kinetics of colony growth and the dependence from the PHA-, I1-2-, agar- and 2-mercaptoethanol concentration are shown. The colony forming cells are identified as T-lymphocytes by usual morphology and by an indirect immunoperoxidase method using mouse anti-Thy 1.2 antibodies.