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Related Experiment Videos

Characterization of a beta-lactamase from Pasteurella multocida.

A Philippon1, B Joly, D Reynaud

  • 1CHU Cochin, Paris, France.

Annales De L'Institut Pasteur. Microbiologie
|March 1, 1986
PubMed
Summary

A bovine Pasteurella multocida strain produces a beta-lactamase enzyme, conferring high resistance to amoxicillin and ticarcillin. This enzyme exhibits a TEM-substrate profile, indicating potential for broad-spectrum antibiotic resistance in veterinary pathogens.

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Area of Science:

  • Veterinary Microbiology
  • Antimicrobial Resistance
  • Enzymology

Background:

  • Bovine Pasteurella multocida is a significant veterinary pathogen.
  • Beta-lactamase production is a key mechanism of antibiotic resistance.
  • Understanding resistance mechanisms in animal pathogens is crucial for effective treatment.

Purpose of the Study:

  • To characterize the beta-lactamase produced by a bovine Pasteurella multocida strain.
  • To determine the enzyme's substrate profile and resistance conferral.
  • To investigate the enzyme's characteristics, including location and genetic relatedness.

Main Methods:

  • Isolation and identification of Pasteurella multocida from bovine samples.
  • Determination of minimal inhibitory concentrations (MICs) for beta-lactam antibiotics.

Related Experiment Videos

  • Enzyme assays to characterize substrate specificity and inhibition patterns.
  • Analysis of enzyme location (periplasmic) and production (constitutive).

    • Main Results:

    • The bovine P. multocida strain exhibited high MICs for amoxicillin (128 mg/l) and ticarcillin (512 mg/l).
    • A periplasmic beta-lactamase was constitutively produced, with no apparent permeability barrier.
    • The enzyme displayed a TEM-substrate profile but was not inhibited by anti-TEM-1 or anti-TEM-2 antisera.
    • The isoelectric point of the enzyme was estimated at 8.8.

    Conclusions:

    • The identified beta-lactamase contributes significantly to amoxicillin and ticarcillin resistance in this bovine P. multocida strain.
    • The TEM-substrate profile suggests a potential link to known beta-lactamase families, despite lack of serological inhibition.
    • Further investigation into the genetic basis of this beta-lactamase is warranted to understand its evolution and spread in veterinary settings.