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Simple rosette assay for HLA-B27 typing of whole blood samples
1Institute of Medical Biology, School of Medicine, University of Tromsø, Norway.
Tissue Antigens
|November 1, 1987
Abstract:
Two human IgM monoclonal antibodies (TrBH12 and Tr3B6) specific for HLA-B27 were coupled to magnetic monodisperse polymer beads by spontaneous adsorption. A few minutes after mixing the beads with 50 microliter of whole blood, rosettes had formed specifically with leucocytes from HLA-B27 positive individuals. In this simple way, reliable typing for HLA-B27 could be conducted rapidly (in about 20 min) by evaluation of rosettes with an inverted microscope and ordinary light. The method requires purified monoclonal antibodies.