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Corneal storage at room temperature.

U Sachs, K Goldman, J Valenti

    Archives of Ophthalmology (Chicago, Ill. : 1960)
    |June 1, 1978
    PubMed
    Summary
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    McCarey-Kaufman medium (M-K medium) better preserves corneal endothelium than moist chambers during room temperature storage. M-K medium protects tissue where metabolic activity resumes, improving endothelial viability after warming.

    Area of Science:

    • Ophthalmology
    • Tissue Preservation
    • Corneal Science

    Background:

    • Short-term eye banking relies on moist chamber and McCarey-Kaufman medium (M-K medium) for preservation at 4°C.
    • Warming preserved corneas to room temperature significantly impacts endothelial cell viability.

    Purpose of the Study:

    • To evaluate the efficacy of M-K medium versus moist chamber preservation for corneas stored at room temperature.
    • To assess the impact of temperature changes on corneal endothelial cell integrity.

    Main Methods:

    • Enzymatic staining and histological examination of rabbit and human corneas.
    • Corneas were stored in M-K medium or moist chambers and subjected to varying temperature conditions (4°C and 25°C).
    • Endothelial viability and organelle integrity were assessed over time.

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    Main Results:

    • Rabbit corneas in M-K medium showed more normal endothelium than moist chamber eyes after seven days at room temperature.
    • Human corneas stored at 4°C for 24 hours and then warmed to 25°C exhibited organelle destruction within 6-12 hours.
    • M-K medium preserved corneal endothelium relatively intact for four days, with some disruption by day seven at room temperature.

    Conclusions:

    • McCarey-Kaufman medium offers superior protection to corneal endothelium compared to moist chambers when stored corneas are warmed to room temperature.
    • M-K medium supports resumed metabolic activity, thereby enhancing tissue viability after temperature shifts relevant to transplantation procedures.