Amine-Functionalized MoO₃@RGO Nanohybrid-Based Biosensor for Breast Cancer Detection

Area of Science:

• Nanomaterials Science
• Biomedical Engineering
• Analytical Chemistry

Background:

• Early and accurate breast cancer detection is crucial for patient outcomes.
• Current diagnostic methods can be invasive, time-consuming, or lack sensitivity.
• Biosensors offer a promising alternative for rapid, sensitive, and specific disease detection.

Purpose of the Study:

• To develop an ultrasensitive, rapid, and label-free biosensor for breast cancer detection.
• To utilize molybdenum trioxide (MoO3) anchored onto reduced graphene oxide (RGO) for enhanced detection capabilities.
• To detect human epidermal growth factor receptor-2 (HER-2) in patient serum as a biomarker.

Main Methods:

• Synthesis of MoO3 nanohybrids on RGO via one-pot hydrothermal method.
• Immobilization of monoclonal antibodies (anti-HER-2) onto the MoO3@RGO/ITO electrode using EDC-NHS chemistry.
• Characterization using electron microscopy, X-ray diffraction, and surface area analysis (BET).

Main Results:

• The MoO3@RGO nanohybrid exhibited a surface area 14 times greater than pristine MoO3.
• Electrochemical techniques (CV, DPV, EIS) demonstrated the platform's binding kinetics and activity.
• The immunosensor achieved high sensitivity (13 uA mLng-1cm-2), a broad detection range (0.001-500 ng mL-1), and a low limit of detection (0.001 ng mL-1).

Conclusions:

• The developed MoO3@RGO nanohybrid-based immunosensor is highly effective for sensitive and rapid HER-2 detection.
• This platform shows significant potential for early breast cancer diagnosis using serum samples.
• Results were validated against Enzyme-Linked Immunosorbent Assay (ELISA), confirming reliability.