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Diagnosis of Hirschsprung's Disease by Immunostaining Rectal Suction Biopsies for Calretinin, S100 Protein and Protein Gene Product 9.5
Published on: April 26, 2019
Clinical Diagnostics of Fecal Calprotectin: a Comparative Study of Actim® Calprotectin and LIAISON® Calprotectin
Background:
Fecal calprotectin (fCal) is an important surrogate biomarker of chronic inflammatory bowel disease (IBD): Crohn's disease and ulcerative colitis. Non-invasive, immunochromatographic rapid tests are excellent tools for the real-time monitoring of disease activity and reduce the need for invasive and costly colonoscopy procedures.
Methods:
In this study the accuracy of fCal detection by the semi-quantitative Actim® Calprotectin and the quantitative automated LIAISON® Calprotectin assay was assessed on a panel of clinical stool samples.
Results:
Of the 119 fecal samples tested, Actim® Calprotectin agreed on 94 samples (79.0%) with the reference test. Furthermore, of the positive samples detected with LIAISON® Calprotectin, 94.0% were interpreted as positive by Actim® Calprotectin.
Conclusions:
Actim® Calprotectin is a rapid test that can be utilized for the initial differentiation between negative and positive samples in an outpatient setting, thus helping to limit more laborious quantitative methods to positive samples.
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Diagnostic studies
A colonoscopy is the definitive screening test, distinguishing ulcerative colitis from other colon diseases with similar symptoms. During a colonoscopy test, inflamed mucosa with exudate ulcerations can be observed, and biopsies are taken to determine the histologic characteristics of the...